南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (09): 1297-.

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上皮性卵巢癌中CD133与EMT相关因子表达的关系

俞岚，周蕾，武世伍，宋文庆，承泽农，郭冰沁   

  • 出版日期:2015-09-20 发布日期:2015-09-20

Expressions of CD133, E-cadherin, and Snail in epithelial ovarian cancer and their clinicopathologic
and prognostic implications

  • Online:2015-09-20 Published:2015-09-20

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摘要： 目的探讨上皮性卵巢癌（Epithelial ovarian cancer, EOC）中CD133 蛋白及上皮-间质转化（Epithelial mesenchymal
transition, EMT）相关因子Snail、E-cadherin 蛋白表达的关系及其功能意义。方法采用免疫组织化学ElivisionTM plus 法检测
150 例EOC和50 例良性上皮性卵巢肿瘤中CD133、Snail 和E-cadherin 蛋白的表达情况。结果良性上皮性卵巢肿瘤组织中
CD133、Snail和E-cadherin蛋白的表达阳性率分别为10%、8.0%和70%，在EOC组织中3者表达分别为58.7%、60.7%和32.7%，
差异有统计学意义（P<0.05）。CD133、Snail 和E-cadherin 的表达与EOC的腹腔脏器和淋巴结转移以及FIGO 分期有关（P<
0.01）；E-cadherin蛋白的表达与Snail及CD133 蛋白的表达均呈负相关关系（r分别为-0.545和-0.570，P均<0.01），CD133蛋白的
表达与Snail蛋白的表达呈正相关关系（r=0.599，P<0.01）。Kaplan-Meier生存分析表明，CD133和Snail的过表达均与患者的生
存率有关，阳性表达的患者生存率明显低于阴性者（P<0.05）；而随着E-cadherin蛋白表达水平的降低EOC患者生存率明显下
降（P<0.05）。多因素分析表明FIGO分期、CD133、Snail 和E-cadherin 的表达是影响EOC根治术后患者预后的独立因素（P<
0.05）。结论CD133和Snail表达的升高以及E-cadherin表达的降低与EOC的侵袭、转移和预后等因素相关，这些指标的联合
检测有可能作为评估EOC患者临床预后的重要指标。

Abstract: Objective To explore expressions of CD133, E-cadherin and Snail in hu¬man epithelial ovarian cancer (EOC) and
elucidate their relationship with the clinicopathologic features and prognosis of the patients. Methods The expression of
CD133, E-cadherin and Snail were detected by immunohistochemical staining in 150 specimens of EOC and 50 specimens of
benign ovarian epithelial tumor tissues. Results The positivity rates of CD133, E-cadherin and Snail protein in EOC were
58.7%, 60.7% and 32.7%, respectively, significantly different from the rates in benign epithelial tumor tissues (10%, 8.0%, and
70% , respectively; P<0.05). The expressions of CD133, E-cadherin and Snail in EOC were significantly correlated with
abdominal organ and lymphnode metastases and FIGO stage (P<0.01). E-cadherin expression was inversely correlated with
Snail and CD133 expression (r=-0.545 and -0.570, P<0.01), and the latter two were positively correlated (r=0.599, P<0.01).
Overexpressions of CD133 and Snail and a decreased expression of E-cadherin were all related to a poor prognosis of the
patients (P<0.05). FIGO stage and expressions of CD133, E-cadherin and Snail were all independent prognostic factors of EOC
(P<0.05). Conclusion The expressions of CD133, E-cadherin and Snail are related to lymph node metastasis, clinical stage, and
prognosis of EOC. Combined detection of these indexes provides important evidence for predicting the progression and
prognosis of EOC.