南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (06): 903-.

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RNA干扰抑制CD59对非小细胞肺癌GLC-P细胞株增殖的影响

吴伟东，刘 丹，侯文进，易永盛，王跃军，林苇嘉   

  • 出版日期:2015-06-20 发布日期:2015-06-20

Effect of RNA interference of CD59 gene on proliferation of non-small cell lung cancer
cell line GLC-P in vitro

  • Online:2015-06-20 Published:2015-06-20

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摘要： 目的运用RNAi技术抑制CD59基因的表达，观察其对非小细胞肺癌细胞株GLC-P增值、凋亡的影响。方法合成可抑
制CD59基因的片段，并运用RNA干扰技术，构建重组质粒，通过脂质体转染法将重组质粒转染至非小细胞肺癌细胞株GLC-P，
并筛选出稳定表达细胞后，采用PCR、MTT、ELISA 法检测该稳定表达细胞株的增值、凋亡所受到的影响。结果干扰后的
GLC-P细胞株中CD59 mRNA表达量较未干扰组显著降低（P<0.05），同时MTT、ELISA实验检测显示肺癌GLC-P细胞增殖能
力降低，可诱导肺癌细胞的凋亡。结论肺癌患者肿瘤细胞中存在CD59高表达，抑制CD59基因表达的同时可抑制GLC-P细
胞的增殖能力并诱导细胞凋亡，为非小细胞肺癌的靶向治疗提供了新靶点、新思路。

Abstract: Objective To investigate the effect of CD59 gene inhibition mediated by RNA interference on the proliferation and
apoptosis of non-small cell lung cancer (NSCLC) GLC-P cells in vitro. Methods Recombinant plasmids for RNA interference of
CD59 gene were constructed and transfected into GLC-P cells via lipofectamine 2000. The stably transfected cells were
examined with real-time RT-PCR, MTT assay and enzyme-linked immunosorbent assay to investigate the changes in cell
proliferation and apoptosis. Results Compared with the control cells, the cells transfected with CD59-siRNA showed
significantly decreased expression levels of CD59 mRNA (P<0.05) and significantly inhibited cell proliferation. Conclusions
CD59 gene is highly expressed in NSCLC and RNA interference-mediated CD59 silencing can strongly inhibit the proliferation
and induce apoptosis in GLC-P cells, which shed light on a potentially new target for targeted gene therapy of NSCLC.