南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (04): 587-.

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解旋酶DDX41在人牙髓组织及牙髓细胞中的表达

杨小军，侯晋，李心竹，胡娇   

  • 出版日期:2015-04-20 发布日期:2015-04-20

Expression of helicase DDX41 in human dental pulp tissues and cells

  • Online:2015-04-20 Published:2015-04-20

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摘要： 目的研究解旋酶DDX41在人牙髓组织和细胞中的表达，为深入探讨其在龋病及牙髓病天然免疫应答过程中的作用奠定
基础。方法应用逆转录-聚合酶链反应（RT-PCR）、免疫细胞化学和免疫组织化学技术对人牙髓组织和牙髓细胞中DDX41的
表达和定位进行分析。结果RT-PCR结果显示DDX41 mRNA在人牙髓细胞中有显著表达；免疫细胞化学和免疫组织化学染
色结果显示DDX41在牙髓细胞的胞浆和胞核中皆有表达，但胞核中的表达水平较低；在牙髓组织中，DDX41主要在牙髓组织
表面的成牙本质细胞的胞浆和胞核中皆有表达。结论人牙髓组织中的成牙本质细胞作为牙髓免疫的效应细胞之一，在龋病和
牙髓炎的免疫反应过程中，细胞内可能存在DDX41/STING依赖的TBK1-IRF3-IFN-β信号通路的活化。

Abstract: Objective To detect the expression of D-E-A-D-box polypeptide 41 (DDX41) in human dental pulp tissues and cells.
Methods The mRNA and protein expressions of DDX41 in human dental pulp cells were detected by RT-PCR and
immunocytochemistry, and the expression of DDX41 in human dental pulp tissues was investigated by
immunohistochemistry. Results Strong expressions of DDX41 mRNA and protein were detected in dental pulp cells. In dental
pulp tissues, DDX41 was expressed in the cytoplasm and nucleus of odontoblasts. Conclusion DDX41/STING-dependent
TBK1-IRF3-IFN-β signaling pathway may play a role in innate immune responses of the dental pulp to caries and pulpitis.