Abstract: Objective To study optical intrinsic signal (OIS) imaging of peri-infarct depolarizations (PIDs) in mice and
investigate the influence of knockout of glial fibrillary acidic protein and vimentin on PIDs. Methods GFAP-/-Vim-/- mice and
GFAP+/+Vim+/+ mice were subjected to MCAO by standard intraluminal filament method. The main characteristics of PIDs in 4 h
were studied by 4-wavelength OIS imaging technique. Results PIDs were identified as consistent, red and blue interaction
waves in the cortical reflectance that slowly propagated peripherally from the origin site. There were 5 patterns of PID
propagation, namely rostro-caudal, latero-medial, caudo-rostral, contralateral and medial-lateral. No significant differences
were found in PID frequency, propagation patterns, velocity or duration time between the two groups (P>0.05). Conclusion
The 4-wavelength OIS system allows acquisition of high temporal-spatial resolution color images for analyzing
temporal-spatial characteristics of PIDs in detail. Knockout of GFAP and vimentin do not affect PIDs in 4 h following middle
cerebral artery occlusion.