南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (12): 1733-.

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外周血中血脑屏障损伤新型标记物脑微血管内皮细胞检测方法的建立

李妍,杜蕾,袁霖,陈德喜,邱嘉文,何肖龙,曹虹,黄胜和   

  • 出版日期:2014-12-20 发布日期:2014-12-20

Establishment of a method for detecting peripheral blood circulating brain microvascular
endothelial cells, a novel biomarker for blood-brain barrier injury

  • Online:2014-12-20 Published:2014-12-20

摘要: 目的建立检测人血脑屏障(BBB)损伤新型标记物外周循环血中脑微血管内皮细胞(cBMECs)的实验方法。方法选取
33名AIDS脑病病例与13名健康对照分组实验,应用免疫磁珠吸附AIDS脑病病例组及健康对照组外周血中内皮细胞后涂片,
免疫荧光三重染色法检测涂片中的cBMECs及相关内皮细胞(cECs和EPCs),并观察其与BBB损伤的关系。结果免疫荧光三
重染色法成功检测到细胞型为CD146+/CD133-/S100B+的cBMECs 细胞,CD146+/CD133-/S100B-的cECs 细胞和CD133+/
CD146+/S100B-的EPCs细胞。结果显示,病例组三种细胞含量均高于对照组,差别有统计学意义(cBMECs、cECs、EPCs分别为
t=4.298,P<0.01;t=4.886,P<0.01;t=4.889,P<0.01)。此外,以cBMECs含量与HIV病毒载量做散点图,可见cBMECs含量随病
毒载量增加而增加,且具有统计学意义(r=0.928,P<0.01)。结论用临床病人标本成功建立了在外周血中检测BBB损伤新型标
记物cBMECs的实验方法。BBB损伤病人外周血中cBMECs含量显著高于健康人。新型标记物cBMECs在无创检测BBB损
伤方面具有重要临床价值。

Abstract: Objective To establish a method for detecting circulating brain microvascular endothelial cells (cBMECs), a novel
biomarker of blood-brain barrier (BBB) injury. Methods Blood samples were collected from 33 patients with AIDS encephalitis
and 13 healthy subjects for detection of cBMECs, cECs and EPCs using magnetic affinity isolation and immune identification
technology. Results The numbers of cBMECs, cECs and EPCs were significantly higher in the AIDS patients than in the control
subjects (t=4.298, P<0.01; t=4.886, P<0.01; t=4.889, P<0.01). An significant association was also noted between HIV load and
cBMEC number (r=0.928, P<0.01). Conclusion We have successfully established a method for detecting peripheral blood
cBMECs, which can be of important value in non-invasive assessment of BBB injury.