南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (11): 1684-.

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MsrA在结直肠癌干细胞中的表达及意义

薛芳沁，杨国华，黄若磊   

  • 出版日期:2014-11-20 发布日期:2014-11-20

Expression of methionine sulfoxide reductase in colorectal cancer stem cells in vitro

  • Online:2014-11-20 Published:2014-11-20

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摘要： 目的探讨MsrA 在结直肠癌干细胞中的表达及与结直肠癌发生、发展的关系。方法免疫磁珠分选结直肠癌细胞株
SW480细胞中CD133+/CD44+/ESA+亚群细胞，RT-PCR检测癌细胞、癌干细胞和正常大肠粘膜细胞中MsrA的表达以及MsrA过
表达对VEGF、MMP-13和CXCR4表达的影响，MTT检测过表达MsrA对结直肠癌干细胞增殖的影响。结果癌干细胞中MsrA
的表达水平明显高于癌细胞，癌细胞中MsrA的表达水平明显高于正常大肠粘膜细胞。MsrA过表达会抑制结直肠癌干细胞的
增殖以及下调VEGF、MMP-13和CXCR4的表达。结论MsrA可能通过下调VEGF、MMP-13和CXCR4的表达和抑制细胞增
殖来抑制结直肠癌的发生、发展。

Abstract: Objective To investigate the expression of methionine sulfoxide reductase (MsrA) in colorectal cancer stem cells and
its association with the tumorigenesis and progression of colorectal cancer. Methods The CD133+/CD44+/ESA+ subpopulation of
colorectal cancer cell line SW480 was obtained by magnetic activated cell sorting (MACS). The expression of MsrA, VEGF,
MMP-13 and CXCR4 in the cancer cells, cancer stem cells and normal colon mucosa cells were detected using RT-PCR. The
proliferation of colorectal cancer stem cells was evaluated with MTT assay. Results The expression of MsrA was significantly
higher in cancer stem cells than in the cancer cells and normal mucosa cells. Overexpression of MsrA inhibited the
proliferation of colorectal cancer stem cells and down-regulated the expression of VEGF, MMP-13 and CXCR4. Conclusions
MsrA suppresses the tumorigenesis and progression of colorectal cancer cells possibly by inhibiting cell proliferation and
down-regulating VEGF, MMP-13 and CXCR4.