南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (11): 1642-.
• • 上一篇 下一篇
黄丽霞,刘香,崔丹婷,许乙凯,游文玮,严轶琛,路新卫,刘瑞源
出版日期:
发布日期:
Online:
Published:
摘要: 目的制备一种苯乙烯类菁染料用于细胞活体荧光成像。方法将1,1,2-三甲基-1氢-苯并吲哚和4-吡啶苯甲醛反应得到苯乙烯类菁探针,考察pH对苯乙烯类菁探针荧光性能的影响,检测探针对细胞活性的影响,最后将苯乙烯类菁探针作为探针应用于活细胞成像。结果制备的苯乙烯类菁探针在中性和碱性条件下显示绿色荧光,在酸性条件下荧光发生由绿色变成橙色。该探针用于细胞活体成像荧光信号清晰可见。结论研制出了一种用pH敏感的苯乙烯类菁探针,该探针表现出了较好的细胞穿透性和细胞活体成像性能。
Abstract: Objective To prepare a pH fluorescence probe based on styrylcyanine dyes for live cell imaging. Methods The Probe1 was prepared by reaction of 4-pyridinecarboxaldehyde with 1,1,2-trimethylbenz[e]indole. The influence of pH on thefluorescent properties was examined, and the cell viability was examined using cell counting kit-8. The Probe 1 was used as apH fluorescence probe in living cell. Results Probe 1 emitted green fluorescence under neutral and basic conditions but orangefluorescence under acid condition. Probe 1 selectively stained the cytoplasmic regions of living cells without significantlyaffecting the cell viability. Conclusion The pH-sensitive fluorescent probe prepared based on styrylcyanine possesses goodability of cell membrane permeation for live cell fluorescent imaging.
黄丽霞,刘香,崔丹婷,许乙凯,游文玮,严轶琛,路新卫,刘瑞源. 苯乙烯类菁染料pH荧光探针在细胞荧光成像中的应用及细胞毒性检测[J]. 南方医科大学学报, 2014, 34(11): 1642-.
0 / / 推荐
导出引用管理器 EndNote|Ris|BibTeX
链接本文: https://www.j-smu.com/CN/
https://www.j-smu.com/CN/Y2014/V34/I11/1642