南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (11): 1611-.

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MUC15在肝细胞癌中的表达及临床意义

刘清泉，田泉，张洪勇，郑鑫，姚英民，刘青光   

  • 出版日期:2014-11-20 发布日期:2014-11-20

  • Online:2014-11-20 Published:2014-11-20

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摘要： 目的探讨黏蛋白MUC15 在肝细胞癌中的表达及临床意义，并分析其和患者预后之间的关系。方法应用实时定量
RT-PCR法及Western blot法检测肝细胞系L02、肝癌细胞系HepG2、MHCC-97H、SMMC-7721，以及122例肝癌组织、对应癌旁
非肿瘤组织中MUC15的表达情况；并分析MUC15的表达与相关临床参数及患者预后的关系。结果肝细胞系L02中MUC15
表达水平显著高于肝癌细胞系HepG2、MHCC-97H、SMMC-7721（P<0.05）；MUC15在肝癌组织的表达水平显著低于癌旁非肿
瘤组织（P<0.05），且MUC15 的表达与肿瘤的TNM分期、肝内或淋巴结转移、门静脉癌栓、病理分级等明显相关（P<0.05）；
Kaplan-Meier生存曲线表明MUC15的低表达是肝细胞癌患者预后差的指标。结论MUC15的表达与肝癌患者的临床病理特
征及预后密切相关，很有可能是肝癌患者预后的一个新的预测指标。

Abstract: Objective To investigate the expression of mucin 15 (MUC15) in hepatocellular carcinoma and explore its association
with the prognosis of patients. Methods The expression of MUC15 was detected by quantitative RT-PCR and Western blotting
in liver cell line L02, liver cancer cell lines HepG2, MHCC-97H, and SMMC-7721, and in 122 HCC and corresponding adjacent
non-tumor liver tissues. The association of MUC15 expression in HCC tissues with the clinical parameters and the patients’
survival was analyzed. Results The 1iver cell line L02 showed significantly higher MUC15 expression level than the liver
cancer cell lines HepG2, MHCC-97H, and SMMC-7721 (P<0.05). The expression level of MUC15 was markedly lower in the
HCC tissues than in the adjacent non-tumor liver tissues (P<0.05). MUC15 expression in the HCC tissues was significantly
correlated with the tumor TNM stage, intrahepatic or lymphatic metastasis, portal vein thrombosis and tumor differentiation
(P<0.05). Kaplan-Meier survival analysis suggested that a low MUC15 expression was associated with a poor clinical prognosis
of the patients. Conclusion The expression of MUC15 is correlated with the clinicopathological features and prognosis of HCC
patients, and may potentially serve as a novel prognostic marker for HCC.