南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (10): 1436-.

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中国人群乙型肝炎病毒基因型间重组的鉴定

潘登，周彬，杨洁，侯金林   

  • 出版日期:2014-10-20 发布日期:2014-10-20

Identification of hepatitis B virus intergenotype recombination in Chinese patients

  • Online:2014-10-20 Published:2014-10-20

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摘要： 目的分析中国乙肝病毒（HBV）的基因型间重组情况，寻找新的重组体。方法下载GenBank中所有来自中国的人HBV
全基因组序列，运用片段分型法分析筛选HBV疑似重组序列，并利用生物信息学软件Simplot鉴定重组事件及重组断点。结果
1642条人全长HBV序列中发现755条重组序列，共31种重组体。其中B/C重组体22种676条重组序列；C/D重组体5种75条
重组序列；A/C重组体3种3条重组序列；C/I重组体1种1条重组序列。排除已有文献报道的重组类型，总共发现4种共4条重组
序列。结论在GenBank所有的来自中国地区的HBV全基因组序列中发现31种重组体，其中4种重组体为首次发现，并且C基
因型参与中国地区所有的基因型间重组。

Abstract: Objective To identify the intergenotype recombination of hepatitis B virus (HBV) in Chinese patients and explore
new recombination types of HBV. Methods Complete genome sequences of HBV from Chinese patients were downloaded
from GenBank and analyzed by Fragment-typing method to screen the potential recombinants, and the breakpoints of
recombination were identified by using Simplot program. Results Thirty-one recombination types including 755 recombinants
were identified from 1642 complete HBV genome sequences, including 22 B/C recombination types with 676 recombinants, 5 C/
D recombination types with 75 recombinants, 3 A/C recombination types with 3 recombinants, and 1 C/I recombination type
with 1 recombinant. Conclusion Of the 31 recombination types comprising 755 recombinants, 4 are novel recombination types
found for the first time. All these recombination types identified in this study involve genotype C.