南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (08): 1110-.

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E3 泛素连接酶HERC4在乳腺癌中的表达及临床意义

周晖，石嵘，陈耀武，曾文利，梁桑华，郑文岭，马文丽   

  • 出版日期:2014-08-20 发布日期:2014-08-20

Expression of E3 ligase HERC4 in breast cancer and its clinical implications

  • Online:2014-08-20 Published:2014-08-20

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摘要： 目的研究HERC4在人乳腺癌组织中的表达，探讨其与乳腺癌临床特征的相关性。方法RT-qPCR法检测67例乳腺癌组
织及配对癌旁乳腺组织标本中HERC4的mRNA表达水平；Western blot和免疫组化法检测67例乳腺癌组织及配对癌旁乳腺组
织标本中HERC4的蛋白表达水平。结果RT-qPCR结果显示HERC4的mRNA的表达水平在乳腺癌组织中较癌旁乳腺组织高
（P<0.05）。Western blot结果显示HERC4蛋白在乳腺癌组织中高表达（P<0.05）。免疫组化结果表明HERC4主要在细胞质中
表达。乳腺癌组织中HERC4阳性表达率为61.2%，癌旁乳腺组织阳性表达率为19.4%。HERC4的表达与乳腺癌TNM分期和
组织学分级密切相关（P<0.05）。结论HERC4与乳腺癌的发生和发展存在相关关系，并有可能作为乳腺癌早期诊断标志物和
治疗靶点。

Abstract: Objective To investigate the expression of HERC4 in human breast cancer tissues and its relationship with the
clinicopathological features. Methods RT-qPCR was used to detect mRNA expression of HERC4, and Western blotting and
immunohistochemistry were employed to detect protein expression of HERC4 in 67 breast cancer tissues and adjacent breast
tissues. Results The results of RT-qPCR showed a significantly higher mRNA expression of HERC4 in breast cancer tissues
than in the adjacent breast tissues (P<0.05). Western blotting demonstrated HERC4 over-expression in breast cancer tissues
compared with the adjacent breast tissues (P<0.05). Immunohistochemistry revealed HERC4 expression located predominantly
in the cell cytoplasm. Positive HERC4 expression was detected in 61.2% of the breast cancer tissues as compared to 19.4% in
the adjacent breast tissues, and its expression level was closely correlated with TNM stage and the histological grade (P<0.05).
Conclusion HERC4 is correlated with the tumorigenesis and progression of breast cancer and may serve as a potential
biomarker for early diagnosis and also as a potential therapeutic target in breast cancer.