南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (08): 1079-.

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准分子激光原位角膜磨镶术后1 年内泪液中基质金属蛋白酶-9的动态变化

姬红培，陈爱蔚，张唯伟，顾荭，张志玲，付举琴   

  • 出版日期:2014-08-20 发布日期:2014-08-20

Dynamic changes of tear fluid matrix metralloproteinase-9
within 1 year after laser in situ keratomileusis

  • Online:2014-08-20 Published:2014-08-20

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摘要： 目的探讨准分子激光原位角膜磨镶术（LASIK）后1 年内基质金属蛋白酶-9（MMP-9）在人眼泪液中的动态变化。方法
收集无明显并发症的32 例LASIK患者术前及术后7、14 d 和1、2、3、6、12 月的泪液，应用免疫印迹技术检测MMP-9 的浓度变
化。结果LASIK术后泪液中MMP-9的浓度呈动态变化，且与术后时间的长短密切相关。术后14 d泪液中MMP-9浓度达到高
峰，约为术前的2.7倍；随后逐渐下降，但在术后12个月MMP-9仍未恢复术前水平，约为术前的1.38倍。结论LASIK术后泪液
中MMP-9的浓度呈时间依赖性变化，术后14 d达到高峰，随后下降，但术后1年仍未恢复至术前水平，提示LASIK术后的角膜
创伤修复反应至少持续1年。

Abstract: Objective To investigate the dynamic changes of matrix metalloproteinase-9 (MMP-9) level in tear fluid within 12
months after laser in situ keratomileusis (LASIK). Methods Twenty-two myopic patients undergoing uneventful LASIK were
enrolled in this study. Tear fluid samples were collected from the patients for measurements of MMP-9 level using Western
blotting preoperatively, at 7 and 14 days, and at 1, 2, 3, 6, and 12 months after the surgery. Results MMP-9 concentrations in
the tear fluid of post-LASIK patients showed a time-dependent variation pattern. MMP-9 reached its peak level in the tear
fluid at 14 days postoperatively, which was 2.70 times the preoperative level; it gradually decreased thereafter but was still 1.38
times the preoperative level at 12 months after the surgery. Conclusions MMP-9 concentrations in the tear fluid of post-LASIK
patients show a time-dependent variation pattern and remains higher than the preoperative level even at 12 months after the
surgery, suggesting that corneal wound healing after LASIK lasts for more than 12 months.