南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (07): 956-.

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坏死细胞逆转脓毒症小鼠Treg/Th17比例的失衡

顾冬生，熊露，韩艳萍，孙尔维   

  • 出版日期:2014-07-20 发布日期:2014-07-20

Transfusion of necrotic cells redresses regulatory T cell and Th17 cell imbalance in septic
mice

  • Online:2014-07-20 Published:2014-07-20

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摘要： 目的研究预输注坏死细胞对脓毒症小鼠模型Treg/Th17细胞平衡的影响。方法将34只C57BL/6小鼠随机分成4组：正
常对照组（PBS组，n=5）、假手术对照组（Sham组，n=5）、脓毒症组（CLP组，n=12）及坏死细胞处理组（Nec组，n=12）。PBS组为
正常小鼠，腹腔注射200 μl PBS一次，Sham组小鼠手术分离盲肠末端后，不进行结扎穿孔；CLP组小鼠用盲肠结扎穿孔法（CLP）
建立脓毒症模型；Nec组小鼠预先腹腔注射2×107个坏死细胞，第5天再行盲肠结扎穿孔。所有小鼠于制备CLP模型后2周处
死，流式细胞仪检测外周血、脾脏和胸腺中CD4+Foxp3+ Treg细胞以及CD4+IL17A+ Th17细胞比例。结果CLP组脾脏中Th17
比例及Treg/Th17比值均显著高于PBS组及sham组（P<0.01）；CLP组小鼠胸腺内Treg比例显著低于Sham组（P<0.01）；而预输
注坏死细胞，可逆转上述CLP手术后小鼠体内Th17比例、Treg比例及Treg/Th17的比例失衡（P<0.05）。结论预输注坏死细胞
可以逆转脓毒症小鼠体内Treg/Th17细胞亚群的失衡。

Abstract: Objective To investigate the effect of transfusion of necrotic cells on regulatory T (Treg) and Th17 cell balance in
septic mice. Method Thirty-four C57BL/6 mice were randomized into PBS group (n=5), sham-operated group (n=5), sepsis
group (n=12), and necrotic cell transfusion group (n=12) and subjected to intraperitoneal PBS injection, sham operation by
separating the cecum only, cecal ligation and puncture (CLP), and injection of 2 × 107 necrotic cells 5 days before CLP,
respectively. All the mice were sacrificed 2 weeks after CLP for analyzing the proportion of CD4+Foxp3+Treg cells and CD4+
IL17A+ Th17 cells in the peripheral blood, spleen and thymus by flow cytometry. Results The percentage of Th17 cells and Treg/
Th17 ratio in the spleen was significantly higher in CLP group than in the sham-operated group and PBS group (P<0.01). The
percentage of Treg cells in the thymus was significantly lower in CLP group than in the sham-operated group (P<0.01).
Pre-infusion of necrotic cells redressed the abnormality of Treg and Th17 cell percentages and Treg/Th17 imbalance in mice
following CLP (P<0.05). Conclusion Pre-infusion of necrotic cells can reverse Treg/Th17 imbalance in septic mice.