南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (05): 699-.

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硫化氢对人结肠癌细胞增殖和迁移的影响

洪敏，唐小卿，何葵   

  • 出版日期:2014-05-20 发布日期:2014-05-20

Effect of hydrogen sulfide on human colon cancer SW480 cell proliferation and migration
in vitro

  • Online:2014-05-20 Published:2014-05-20

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摘要： 目的研究H2S 对人结肠癌SW480 细胞增殖和迁移的作用，及其调控的分子机制。方法用不同浓度H2S 处理结肠癌
SW480后，采用MTT法检测结肠癌SW480细胞增殖能力的改变，细胞划痕实验观察SW480细胞迁移能力的改变，Western blot
实验检测MMP-2、MMP-9和SIRT1表达水平的改变。结果MTT实验，24、48、72 h 3个时间组50、100、200、400 μmol/L H2S处
理后，与相应时间对照组相比，H2S均能促进细胞的增值；细胞迁移实验结果显示NaHS（100 μmol/l）处理组迁移能力增强且明
显高于正常对照组（P<0.01），Western blot结果显示NaHS（100 μmol/l）处理组MMP-2和MMP-9的表达水平增强且明显高于正
常对照组（P<0.05，0.01），且NaHS（100 μmol/l）处理组SIRT1表达水平增强。结论H2S能够促进人结肠癌SW480细胞的增殖和
迁移，其机制可能与上调SIRT1的表达有关。

Abstract: Objective To investigate the effect of hydrogen sulfide (H2S) on the proliferation and migration of human colon
cancer SW480 cells and explore its molecular mechanisms. Methods The proliferation of SW480 cells exposed to different
concentrations of NaHS for varying time lengths was analyzed by MTT assay, and the changes in cell migration was evaluated
using wound-healing assay. The changes in the expression levels of MMP-2, MMP-9 and SIRT1 protein were detected by
Western blotting in the exposed cells. Results Compared with the control cells, SW480 cells exposed to 50, 100, 200, or 400
μmol/L NaHS for 24, 48 and 72 h all showed increased proliferative activity. NaHS treatment at 100 μmol/L significantly
promoted the cell migration (P<0.01) and enhanced the cellular expressions of MMP-2 (P<0.05) and MMP-9 (P<0.01) proteins;
NaHS exposure (100 μmol/L) also resulted in up-regulation of SIRT1 expression in SW480 cells. Conclusion H2S can promote
proliferation and migration of SW480 cells in vitro, the mechanism of which may involve up-regulated expression of SIRT1.