南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (05): 686-.

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槲皮素诱导U87细胞凋亡及对MDM2-p53负反馈调节的影响

王惠丽，苑召虎，陈志江，姚芳，胡子有，吴炳义   

  • 出版日期:2014-05-20 发布日期:2014-05-20

Effect of quercetin on glioma cell U87 apoptosis and feedback regulation of MDM2-p53

  • Online:2014-05-20 Published:2014-05-20

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摘要： 目的探讨槲皮素对人脑胶质瘤U87 细胞凋亡及MDM2-p53 负反馈调节的影响。方法以不同浓度槲皮素（50、100、
150 μmol/L）处理U87细胞，分别采用流式细胞术、RT-PCR及Western blotting技术检测槲皮素对U87细胞凋亡的影响、MDM2
mRNA及p53、caspase-3 蛋白的表达。结果槲皮素处理U87 细胞48 h 后，流式细胞术结果显示细胞凋亡率：Q50组为（22.53±
0.72）%，Q100组为（29.06±0.81）%，Q150组为（31.5±0.45）%，明显高于对照组（12.40±0.70）%（P<0.05）。随着槲皮素浓度的升高，泛
素连接酶MDM2 mRNA表达量升高，在蛋白水平发现，槲皮素处理后，caspase-3凋亡蛋白表达增加，p53蛋白的表达降低。结
论槲皮素可以促进人脑胶质瘤U87细胞凋亡，通过凋亡蛋白caspase-3发挥作用，并且在凋亡过程中可以发生MDM2-p53的负
反馈调节。

Abstract: Objective To investigate the effect of quercetin on apoptosis and feedback regulation of MDM2-p53 in multiform
glioblastoma U87 cells in vitro. Methods U87 cells exposed to different concentrations of quercetin (50, 100, and 150 μmol/L)
were examined with flow cytometry, RT-PCR and Western blotting for detecting the cell apoptosis, MDM2 mRNA expression,
and p53 and caspase-3 expressions. Results Quercetin induced obvious apoptosis in U87 cells in a concentration-dependent
manner, with apoptosis rates of (12.40 ± 0.70)% at Q0, (22.53 ± 0.72)% at Q50, (29.06 ± 0.81)% at Q100, and (31.5 ± 0.45)% at Q150.
Quercetin significantly increased the expressions of MDM2 mRNA and active caspase-3 protein but decreased the expression
of p53 in the cells. Conclusion Quercetin promotes the apoptosis of multiform glioblastoma U87 cells mediated by caspase-3
and influences the feedback balance of MDM2-p53.