南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (04): 552-.

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子痫前期患者细胞间粘附分子-1 K469E及血小板内皮细胞粘附分子-1 C373G的基因多态性

何艳红，王志坚，靳瑾，朱晓雯，陈玉英，陈淑滢，曹艳文   

  • 出版日期:2014-04-20 发布日期:2014-04-20

Polymorphisms of intercellular adhesion molecule-1 gene K469E and platelet endothelial
cell adhesion molecule-1 gene C373G in patients with preeclampsia

  • Online:2014-04-20 Published:2014-04-20

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摘要： 目的探讨细胞间粘附分子-1（ICAM-1）基因K469E多态性及血小板内皮细胞粘附分子-1（PECAM-1）基因C373G多态性
各等位基因及基因型在子痫前期患者及健康孕妇中的分布频率，初步分析上述基因多态性与子痫前期的关系。方法采用聚合
酶链反应-限制性片段多态性（PCR-RFLP）方法和DNA序列测序法对110名子痫前期患者及110名健康足月孕妇ICAM-1基因
K469E位点及PECAM-1基因C373G位点进行检测，同时比较两组人群中的相关的临床资料。结果ICAM-1基因K469E位点
及PECAM-1基因C373G位点基因频率分布符合Hardy-Weinberg平衡定律，具有群体代表性。ICAM-1基因K469E位点基因
型频率及等位基因频率在两组人群中均无显著差异（P>0.05）。PECAM-1基因C373G位点CC基因型和CG基因型在两组人群
分布中存在显著差异（P<0.05）。CG基因型携带者患子痫前期的风险是CC基因型的1.959倍（OR=1.959，95%CI:1.090-3.520，
P=0.024），且通过Logistic回归分析发现该相关性独立于孕妇年龄、孕次、产次及孕前BMI。C等位基因频率和G等位基因频率
在两组人群的分布差异无统计学意义（P>0.05）。结论PECAM-1基因C373G位点CG基因型携带者可能与子痫前期患者较高
的遗传易感性相关，而ICAM-1基因K469E多态性则与子痫前期的发病无相关。

Abstract: Objective To investigate the role of Sam68 (Src-associated substrate during mitosis 68 kD) in the occurrence and
development of colorectal cancer. Methods Colorectal cancer cell lines with stable Sam68 over-expressing and low Sam68
expression were established to test the effect of Sam68 in the proliferation, invasion, and migration of the cancer cells using
colony formation, MTT and Transwell assays. Results SW480 and Ls174t colorectal cell lines over-expressing Sam68 showed
significantly enhanced cell proliferation, invasion and migration (P<0.05). Conversely, the low Sam68 expression in SW620 and
HCT116 colorectal cell lines significantly suppressed the cell proliferation, invasion and migration (P<0.05). Conclusion The
expression of Sam68 can promote the proliferation, invasion and migration of colorectal cancer cells lines in vitro.