南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (04): 507-.

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SCF/c-Kit信号促进膀胱癌T24细胞侵袭作用

郭术俊，陶象男，汪忆梦，唐洁，申林，宋传旺   

  • 出版日期:2014-04-20 发布日期:2014-04-20

SCF/c-Kit signaling promotes invasion of T24 cells via PI3K pathway

  • Online:2014-04-20 Published:2014-04-20

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摘要： 目的探讨SCF/c-Kit信号对膀胱癌T24细胞侵袭性的影响。方法采用Western blot检测T24细胞c-Kit表达及经SCF刺
激后PI3K 途径激活情况；Transwell 侵袭实验（直接与间接计数法）观察T24 细胞经过SCF（1 ng/ml）刺激前后及使用
Wortmannin（特异性PI3K阻断剂）预处理后侵袭性的变化。结果T24细胞存在c-Kit蛋白的表达并且经SCF（1 ng/ml）作用24 h
后，出现明显的磷酸化Akt；与对照组相比，SCF（1 ng/ml）刺激组穿过聚碳酸酯膜的T24 细胞数明显增多（P<0.01），而
Wortmannin预刺激阻断了SCF的这种效应。结论SCF/c-Kit信号促进了T24细胞的侵袭性，这一效应是由PI3K途径介导的。

Abstract: Objective To explore the role of SCF/c-Kit signaling in the invasion of bladder cancer T24 cells. Methods Western
blotting was used to detect the expression of c-Kit and PI3K pathway activation stimulated by stem cell factor (SCF) in T24
cells. The invasiveness of T24 cells before and after SCF stimulation and Wortmannin (aspecific PI3K inhibitor) treatment was
evaluated using Transwell invasion assay (direct and indirect counting methods). Results T24 cells expressed c-Kit protein and
showed obvious Akt phosphorylation after stimulation with SCF (1 ng/ml) for 24 h. Compared to the control group, SCF
stimulation (1 ng/ml) caused a greater number of T24 cells to migrate through the polycarbonate film (P<0.01), and this effect
was blocked by the application of Wortmannin before the stimulation. Conclusion SCF/c-Kit signaling promotes the
invasiveness of T24 cells, and this effect is mediated by the PI3K pathway.