南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (04): 492-.

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活性维生素D3下调屋尘螨诱导的气道上皮细胞胸腺基质淋巴生成素蛋白释放

周丽芹，董航明，赵海金，邹梦晨，姚利红，邹飞，蔡绍曦   

  • 出版日期:2014-04-20 发布日期:2014-04-20

1,25-dihydroxyvitamin D3 pretreatment inhibits house dust mite-induced thymic stromal
lymphopoietin release by human airway epithelial cells

  • Online:2014-04-20 Published:2014-04-20

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摘要： 目的探讨屋尘螨（HDM)对气道上皮细胞系（16-HBE）胸腺基质淋巴生成素（TSLP）表达的影响，进一步评价活性维生素
D3（1,25VD3）的干预作用。方法四唑盐（MTT）比色法检测不同浓度HDM（200、400、800 U/L）及其联合10-8 mol/L 1,25VD3对
16-HBE活力的影响。按下述分组刺激16-HBE，对照组，400 U/L HDM组，10-8 mol/L 1,25VD3 组，10-8 mol/L 1,25VD3 预处理
1 h后400 U/L HDM组，10-8 mol/L 1,25VD3联合400 U/L HDM组，6 h后，实时荧光定量PCR检测细胞TSLPmRNA表达情况；
刺激24 h 后，ELISA 检测细胞培养上清中TSLP 的浓度。结果200~800 U/L HDM作用气道上皮细胞后，可以上调16-HBE
TSLP的基因和蛋白表达（P<0.05）；10-8 mol/L 1,25VD3预处理后400 U/L HDM组的TSLP蛋白水平较400 U/L HDM组显著下
降（P<0.05）；但10-8 mol/L 1,25VD3 联合400 U/L HDM组的TSLP 蛋白水平较400 U/L HDM组无显著差异（P=0.58）。结论
HDM和1,25VD3均可增加16-HBE TSLP的表达和释放。1,25VD3预处理气道上皮细胞可减少屋尘螨诱导的TSLP分泌。

Abstract: Objective To investigate the effect of 1,25-dihydroxyvitamin D3 (1,25VD3) on house dust mites (HDM)-induced
expression of thymic stromal lymphopoietin (TSLP) in human airway epithelial cells in vitro. Methods Human airway
epithelial 16HBE cells were incubated with 200, 400, and 800 U/L in the absence or presence of 1,25VD3 (10-8 mol/L) for 6 h and
24 h, and TSLP mRNA and protein expressions in the cells were assessed using quantitative PCR and ELISA. Results 16HBE
cells incubated with HDM at 200, 400, and 800 U/L showed significantly increased TSLP mRNA and protein expressions (P<
0.05). Pretreatment of the cells with 1,25VD3 obviously lowered 400 U/L HDM-induced TSLP expressions (P<0.05), but 1,25VD3
added along with HDM in the cells did not produce significant effects on TSLP expressions (P=0.58). Conclusion Both 1,25VD3
and HDM can induce TSLP expression and release in 16HBE cells, but pretreatment with 1,25VD3 can decrease
HDM-augmented TSLP expression in the cells.