南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (03): 410-.

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IL-1β诱导子宫内膜异位症间质细胞activin A及相关因子的表达

李蕾，刘木彪，朱峰城，潘颖   

  • 出版日期:2014-03-20 发布日期:2014-03-20

Effect of interleukin-1β on expressions of activin A and its related factors in cultured
endometrial stromal cells from patients with endometriosis

  • Online:2014-03-20 Published:2014-03-20

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摘要： 目的探讨白细胞介素1β（IL-1β）对子宫内膜异位症（内异症）在位内膜间质细胞activin A、follistatin、cripto的表达及蛋白
分泌的影响。方法分离培养内异症在位内膜间质细胞，加入不同浓度IL-1β（250 pg/ml，500 pg/ml，750 pg/ml）干预。实时荧光
定量PCR技术及酶联免疫吸附试验，分别从mRNA及蛋白水平检测activin A、follistatin、cripto 的变化。结果经IL-1β刺激，
activin A及follistatin mRNA表达及蛋白分泌较对照组呈IL-1β剂量依赖性增加；cripto mRNA表达较对照组呈IL-1β剂量依赖
性增加。结论IL-1β可影响内异症在位内膜activin A、follistatin、cripto的分泌及表达。

Abstract: Objective To study the effect of interleukin-1β (IL-1β) on the expressions activin A, follistatin, and cripto in cultured
human endometrial stromal cells (HESCs) form patients with endometriosis. Methods Cultured HESCs were stimulated with
250, 500, and 750pg/ml IL-1β, and the mRNA and protein expressions of activin A, follistatin, and cripto were assayed using
real-time reverse transcriptase–polymerase chain reaction and enzyme-linked immunosorbent assay. Results IL-1β treatment
caused significant dose-dependent increments of the mRNA and protein expressions of activin A and follistatin and of the
mRNA expression of cripto in cultured HESCs. Conclusion IL-1β can affect the expressions of activin A, follistatin and cripto
in HESCs from patients with endometriosis.