南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (02): 218-.

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miR-221调控PTEN/Akt信号介导人胶质瘤耐药细胞上皮间质转化相关基因的表达

谢强，黄作平，闫雍容，李锋，钟雪云   

  • 出版日期:2014-02-20 发布日期:2014-02-20

MiR-221 mediates epithelial-mesenchymal transition-related gene expressions via regulation
of PTEN/Akt signaling in drug-resistant glioma cells

  • Online:2014-02-20 Published:2014-02-20

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摘要： 目的探讨miR-221在耐药胶质瘤细胞中与上皮间质转化的相关性。方法荧光定量PCR和蛋白免疫印迹分析方法比较
人胶质瘤细胞株Z1（原发性耐药细胞株）、Z2（药物敏感细胞株）和Z2-BCNU（耐药细胞株）中miR-221、PTEN、p-Akt、
E-cadherin、vimentin、MRP1 表达的差异。结果PTEN在Z2 细胞中蛋白表达含量明显高于miR-221、vimentin 高表达的Z1 和
Z2-BCNU细胞，在E-cadherin高表达的Z2细胞中vimentin、p-Akt和MRP1含量明显减低。结论MiR-221通过下调PTEN激活
PI3-K/Akt信号从而调控上皮间质转化相关基因的表达。

Abstract: Objective To investigate the correlation between miR-221 and epithelial-mesenchymal transition (EMT) in
drug-resistant glioma cells. Methods The expression levels of miR-221, PTEN, p-Akt, E-cadherin, vimentin, and MRP1 were
quantitatively analyzed in Z1 cells (primary drug-resistant cells), Z2 cells (drug-sensitive cells) and Z2-BCNU cells
(drug-resistant cells) using fluorescent real-time PCR and Western blotting. Results The expression levels of PTEN were
significantly increased in Z2 cells compared with Z1 and Z2-BCNU cells which overexpressed miR-221 and vimentin. The
expression levels of vimentin, p-Akt and MRP1 were significantly decreased in Z2 cells overexpressing E-cadherin.
Conclusion MiR-221 regulates the expression of EMT-related genes through down-regulation of PTEN and activation of PI3-K/
Akt signaling.