南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (02): 164-.

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miR-520a调控ErbB4的表达并抑制食管鳞癌细胞的增殖与侵袭

叶文广，姚青林，张明鑫，闻勤生，王景杰   

  • 出版日期:2014-02-20 发布日期:2014-02-20

miR-520a regulates ErbB4 expression and suppresses proliferation and invasion of
esophageal squamous cell carcinoma

  • Online:2014-02-20 Published:2014-02-20

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摘要： 目的探讨miR-520a对ErbB4的调控及其对食管鳞癌生物学行为的影响。方法应用分子生物学技术构建miR-520a的真
核表达质粒、ErbB4的野生型及突变性3’-UTR报告基因，报告基因分析miR-520a对ErbB4的调控，蛋白印迹检测转染miR-520a
对ErbB4蛋白表达水平的影响。应用MTT、Transwell侵袭试验探讨miR-520a对食管鳞癌细胞株增殖、凋亡及侵袭等生物学行
为的影响。结果报告基因分析显示miR-520a能够下调ErbB4的野生型3’-UTR报告基因的荧光表达，而对突变性报告基因的
荧光表达影响不大。蛋白印迹显示miR-520a可以显著抑制ErbB4的蛋白表达水平。此外，miR-520a可以显著抑制细胞增殖并抑
制侵袭。结论miR-520a调控ErbB4的表达并能够抑制食管鳞癌细胞的增殖与侵袭，miR-520a在食管鳞癌中发挥抑癌基因功能。

Abstract: Objective To investigate the role of miR-520a in regulation ErbB4 expression and the biological behavior of
esophageal squamous cell carcinoma (ESCC). Methods The role of miR-520a in regulating the expression of ErbB4 was
investigated by Western blotting and luciferase reporter assay system. The effect of miR-520a on the proliferation and invasion
of ESCC cells was detected by MTT and Transwell invasion assay, respectively. Results Western blotting and luciferase
reporter assay revealed that miR-520a down-regulated the expression of ErbB4 in vitro. miR-520a significantly inhibited the
proliferation and suppressed the invasion of ESCC cell line Eca109. Conclusion miR-520a regulates the expression of ErbB4
and suppresses the proliferation and invasion of ESCC cells in vitro, suggesting its role as a tumor suppressor.