南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (12): 1778-.

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STAT3与P-STAT3在转基因AD小鼠脑组织中的表达及意义

田密，侯德仁，邓炎尧，李维，奉夏露   

  • 出版日期:2013-12-20 发布日期:2013-12-20

Expression of STAT3 and P-STAT3 in the brain of a transgenic mouse model of
Alzheimer’s disease

  • Online:2013-12-20 Published:2013-12-20

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摘要： 目的通过检测STAT3及P-STAT3在APPswe/PS△E9双转基因阿尔茨海默病（AD）小鼠脑组织中的表达，探讨其在AD发
病过程中的可能作用。方法采用免疫组化法检测APPswe/PS△E9双转基因AD小鼠及对照小鼠脑组织中STAT3和P-STAT3
的表达。结果STAT3和P-STAT3表达于脑组织的不同部位。STAT3在转基因AD小鼠和对照小鼠的大脑皮层、基底前脑、海马
及小脑中的阳性表达率分别为93.75%、87.50%，87.50%、43.75%，81.25%、37.50%，62.50%、0.00%，其中差异有统计学意义的是
基底前脑、海马及小脑中的表达（P<0.05）；P-STAT3在转基因AD小鼠和对照小鼠的大脑皮层、基底前脑、海马及小脑中的阳性
表达率分别为0.00%、0.00%，68.75%、0.00%，62.50%、12.50%，43.75%、0.00%，其中差异有统计学意义的是基底前脑、海马及小
脑中的表达（P<0.05）；且STAT3和P-STAT3呈正相关（P<0.05）。结论STAT3和P-STAT3在转基因AD小鼠的基底前脑、海马及
小脑中存在高表达，其可能参与了AD发病的病理过程。

Abstract: Objective To detect the expression of signal transducer and activator of transcription 3 (STAT3) and P-STAT3 in the
brain of the APPswe/PS △E9 double transgenic mouse model of Alzhaimer’s disease (AD) and invesitgate their possible role in
AD. Methods APPswe/PS △E9 double transgenic mice and control mice were examined for cerebral STAT3 and P-STAT3
expressions using immunothistochemistry. Results STAT3 and P-STAT3 were expressed in the different regions of mouse
brain. In the transgenic mice and the control mice, the positivity rates of STAT3 were 93.75% and 87.50% in the cerebral cortex,
87.50% and 43.75% in the basal forebrain, 81.25% and 37.50% in the hippocampus, and 62.50% and 0.00% in the cerebellum,
respectively, showing significant differences between the mice in the STAT3 expressions in the basal forebrain, hippocampus
and cerebellum (P<0.05). The positivity rates of P-STAT3 in the two groups were 0.00% and 0.00% in the cerebral cortex, 68.75%
and 0.00% in the basal forebrain, 62.50% and 12.50% in the hippocampus, and 43.75% and 0.00% in the cerebellum,
respectively, showing also significant differences in the basal forebrain, hippocampus and cerebellum (P<0.05). The expression
of STAT3 was positively correlated with that of P-STAT3 in transgenic AD mice (P<0.05). Conclusion STAT3 and P-STAT3 are
highly expressed in the basal forebrain, hippocampus and cerebellum in transgenic AD mice and may participate in the
pathological process of AD.