南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (12): 1775-.

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软脂酸增加血管内皮细胞Toll样受体4表达及活化TLR4炎症信号通路

赵乃倩，王冬青，荣青峰，韩乐，赵文慧，张策   

  • 出版日期:2013-12-20 发布日期:2013-12-20

Palmitate enhances Toll-like receptor 4 expression and signaling in porcine vascular
endothelial cells

  • Online:2013-12-20 Published:2013-12-20

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摘要： 目的研究饱和脂肪酸软脂酸对血管内皮细胞Toll样受体4（TLR4）表达及信号转导的影响。方法采用软脂酸处理猪髋
动脉内皮细胞（PIECs），实时荧光定量PCR检测PIECs TLR4 mRNA表达，蛋白印迹法检测PIECs TLR4及IκBα蛋白表达，流式
细胞仪检测PIECs细胞表面TLR4蛋白水平，酶联免疫吸附测定法检测细胞培养液TNF-α及IL-6水平。结果与对照组比较，
软脂酸组PIECs TLR4 mRNA及蛋白表达显著升高（TLR4 mRNA：4.73±0.61 vs 1.25±0.90，P<0.05；TLR4 蛋白：5.79±0.05 vs
4.07±0.31，P<0.05），PIECs细胞表面TLR4蛋白水平显著增加（38.070±3.907 vs 29.390±1.072，P<0.05）；软脂酸组PIECs IκBα蛋
白表达显著降低（2.04±0.22 vs 3.98±0.18，P<0.05），细胞培养液TNF-α及IL-6水平显著升高（TNF-α：2.52±0.30 vs 1.38±0.26，P<
0.05；IL-6：3.28±0.32 vs 1.44±0.28，P<0.05）。结论软脂酸在诱导血管内皮细胞TLR4表达的同时活化TLR4炎症信号通路。

Abstract: Objective To study the effect of palmitate on toll-like receptor 4 (TLR4) expression and signaling in vascular
endothelial cells. Methods Pig iliac endothelial cells (PIECs) were incubated with palmitate. TLR4 gene expression levels were
measured by quantitative real-time PCR, and TLR4 and IκBα protein expressions by Western blotting. The expression levels of
TLR4 protein on the surface of PIECs were quantified using flow cytometry. ELISA was employed to detect tumor necrosis
factor-α (TNF-α) and interleukin-6 (IL-6) concentrations in the cell medium. Results Palmitate treatment significantly
increased TLR4 mRNA and protein expression levels in PIECs compared with those in the control cells (4.73±0.61 vs 1.25±0.90,
P<0.05; 5.79±0.05 vs 4.07±0.31, P<0.05). The expression levels of TLR4 on the cell surface significantly increased (38.070±3.907 vs
29.390±1.072, P<0.05), while IκBα protein level was significantly lowered in PIECs after palmitate treatment as compared with
those in the control cells (2.04±0.22 vs 3.98±0.18, P<0.05). Palmitate treatment significantly elevated TNF-α (2.52±0.30 vs 1.38±
0.26, P<0.05) and IL-6 (IL-6: 3.28±0.32 vs 1.44±0.28, P<0.05) concentrations in the cell culture medium. Conclusion Palmitate can
enhance TLR4 expression and signaling in porcine vascular endothelial cells.