南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (12): 1723-.

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补骨脂素可能参与诱导大鼠间充质干细胞向雄性生殖细胞方向分化

万谦，陆华，邓延莉，梁丽梅   

  • 出版日期:2013-12-20 发布日期:2013-12-20

Psoralen induces rat mesenchymal stem cell differentiation towards male germ cells in
vitro

  • Online:2013-12-20 Published:2013-12-20

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摘要： 目的验证补骨脂素能诱导大鼠间充质干细胞向雄性生殖细胞方向分化的假说。方法使用终浓度为3.0 μg/ml的补骨脂
素干预大鼠间充质干细胞，显微观察细胞形态变化情况，接着使用MTT法检测细胞增殖变化，最后使用实时荧光定量PCR
（Qpcr）测定被干预细胞的七种代表性的生殖分化标记基因的表达变化，七种标记基因分别为：Oct-4、Stra8、RVLG、SCP3、TNP2、
Itgb1、Itga6。结果显微观察表明3.0 μg/ml 的补骨脂素干预72 h 后，大鼠间充质干细胞形态变化不显著；MTT法检测表明
3.0 μg/ml的补骨脂素干预的5 d中，大鼠间充质干细胞增殖情况变化不显著（P>0.05）；Qpcr测定表明3.0 μg/ml的补骨脂素干预
72 h 后，显著上调了Oct-4、Stra8、SCP3、Itgb1 等基因的表达（P<0.05），而对RVLG、TNP2、Itga6 等基因的表达影响不显著（P>
0.05）。结论补骨脂素可能参与诱导大鼠间充质干细胞向雄性生殖细胞方向的分化。

Abstract: Objective To test the hypothesis that psoralen induces rat mesenchymal stem cells (RMSCs) to differentiate towards
male germ cells. Methods RMSCs were induced by psoralen at the final concentration of 3.0 μg/ml, and the morphological
changes of the cells were detected microscopically and the cell proliferation changes were measured by MTT assay. The
expressions of 7 representative marker genes of male germ cells, namely Oct-4, Stra8, RVLG, SCP3, TNP2, Itgb1, and Itga6, were
investigated in the induced cells by real-time quantitative PCR. Results RMSCs showed no obvious morphological changes
after induction with 3.0 μg/ml psoralen for 72 h. Psoralen induction for 5 days did not significantly affect the proliferation of
RMSCs; Psoralen induction for 72 h, however, significantly up-regulated the genes Oct-4, Stra8, SCP3, and Itgb1 (P<0.05)
without affecting the expressions of the genes RVLG, TNP2, or Itga6 (P>0.05). Conclusion Psoralen may participate in the
differentiation of RMSCs towards male germ cells.