南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (11): 1628-.

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电穿孔技术对DNA疫苗pVAX-tG250FcGB免疫效果的影响

肖毅，高昆，杨勇，阎瑾琦，张亮，王宇，徐元基，田仁礼，杜芝燕2，于继云   

  • 出版日期:2013-11-20 发布日期:2013-11-20

Influence of electroporation on immunogenicity of the DNA vaccine pVAX-tG250FcGB

  • Online:2013-11-20 Published:2013-11-20

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摘要： 目的探讨应用电穿孔技术对DNA 疫苗pVAX-tG250FcGB 免疫效果的影响。方法构建肾癌DNA 疫苗pVAXtG250FcGB，
通过体内电穿孔技术或普通肌肉注射途径，将DNA疫苗导入到BALB/c小鼠体内，探讨电穿孔免疫途径诱导抗原
特异性免疫应答的效果。结果电穿孔技术或普通肌肉注射均能在小鼠体内诱导出抗原特异性的体液免疫应答和细胞免疫应
答，电穿孔技术的免疫效果明显优于普通肌肉注射途径。结论电穿孔技术介导的DNA疫苗pVAX-tG250FcGB免疫具有较强
的诱导免疫应答的能力，是研究DNA疫苗pVAX-tG250FcGB免疫效果的一种有效途径。

Abstract: Objective To investigate the influence of electroporation on the immunogenicity of the DNA vaccine pVAXtG250FcGB.
Methods The DNA vaccine pVAX-tG250FcGB was constructed by inserting the coding gene of tG250 fusion genes
into the expression vector pVAX. The DNA vaccine was delivered in BALB/c mouse by electroporation or intramuscular
injection, and the induced antigen specific immune responses were compared. Results The vaccine delivered by
electroporation and intramuscular injection both induced immune responses in BALB/c mouse, but electroporation produced
an obviously stronger effect than intramuscular injection. Conclusion Electroporation-mediated DNA vaccine delivery can
produce strong immune response in mice and is an effective means for studying the immunogenic effect of DNA vaccine
pVAX-tG250FcGB.