南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (11): 1596-.

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人UCA1基因转录调控的生物信息学分析与鉴定

薛梅，李旭，陈葳   

  • 出版日期:2013-11-20 发布日期:2013-11-20

Bioinformatics analysis and identification of transcriptional regulation of human UCA1 gene

  • Online:2013-11-20 Published:2013-11-20

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摘要： 目的生物信息学分析与鉴定人类膀胱癌特异性表达的UCA1 基因转录调控作用原理。方法运用CpG岛预测软件
EMBOSS CpGplot、CpG Island Searcher、Methprimer、CpG finder对UCA1基因启动子CpG岛进行分析。利用转录因子预测软
件MatrixCatch和TFSEARCH对UCA1基因核心启动子区可能结合的转录因子进行分析。染色质免疫沉淀技术对所预测到的
转录因子进行分子生物学实验验证。结果CpG岛预测发现UCA1基因全长启动子区中不存在CpG岛，因此UCA1基因属于组
织特异性基因与前期研究报道结果一致。生物信息学预测及筛选发现UCA1基因核心启动子存在4个与肿瘤关系密切的转录
因子。根据预测结果选取2个转录因子利用染色质免疫沉淀实验鉴定，确定1个转录因子可与UCA1基因核心启动子区结合。
结论UCA1 基因启动子区无CpG岛存在，该基因核心启动子区可能与多种转录因子结合，并确定转录因子c-Myb 可以与
UCA1基因核心启动子区结合。

Abstract: Objective To analyze the transcription factor binding sites and methylation in the promoter region of UCA1 gene.
Methods UCA1 gene promoter was analyzed using CpG software to predict the CpG island. MatrixCatch and TFSEARCH
were used to predict the potential transcription factor binding sites in UCA1 gene core promoter. ChIP assay was used to
identify the transcription factors binding to UCA1 gene core promoter. Results UCA1 gene promoter contained no CpG island
and was therefore a typical tissue-specific gene. There were 4 transcription factors associated with human cancers in UCA1
gene core promoter, but only one of them interacted with UCA1 gene core promoter. Conclusion There is no CpG island in
UCA1 gene promoter region, and the transcription factor c-Myb can specifically bind to the core promoter.