南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (11): 1559-.

• •    下一篇

人绒毛外滋养层细胞HTR-8/SVneo 中Toll样受体mRNA的表达及对吲哚胺2,3-双加氧酶mRNA水平的影响

许巍，杨贵波，端家忠，王越，姚文荣，刘学庆，陈雪梅，丁裕斌，王应雄，何俊琳   

  • 出版日期:2013-11-20 发布日期:2013-11-20

Effects of Toll-like receptors on indoleamine 2, 3-dioxygenase mRNA levels in human trophoblast HTR-8/SVneo cells

  • Online:2013-11-20 Published:2013-11-20

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摘要： 目的系统检测人绒毛外滋养细胞HTR-8/SVneo中Toll样受体（TLR）mRNA的表达情况，定量分析TLRs配体刺激前后吲
哚胺2,3双加氧酶（indoleamine 2,3-dioxygenase, IDO）mRNA的表达差异。方法RT-PCR检测HTR-8/SVneo细胞中TLR 1-10
mRNA 的表达情况；实时荧光定量RT-PCR 检测TLR3、TLR4、TLR7/8、TLR9 配体刺激前后IDO mRNA 表达水平。结果
HTR-8/SVneo细胞中有IDO及TLR 1-10 mRNA表达；在48 h内IDO mRNA表达随着时间延长，呈升高趋势；IDO mRNA的表
达与培养基营养状况相关；TLRs配体刺激后，除TLR-3转录水平表达下调外，TLR-4、7、8、9 mRNA表达均上调；poly（I∶C）刺激
后IDO mRNA 表达低于正常组，IFN-γ刺激后表达高于正常组（P<0.05）。结论TLRs mRNA的表达提示该细胞具有抗感染作
用；HTR-8/Svneo细胞中IDO mRNA的表达与母胎界面营养状况相关；TLRs配体刺激剂对TLRs及IDO mRNA表达的影响不
仅验证了TLRs的功能活性，而且提示了IDO在抗病原体免疫应激中可依赖TLRs途径发挥作用。

Abstract: Objective To study the expression of Toll-like receptors (TLRs) mRNA in human trophoblast HTR-8/SVneo cells and
the changes in indoleamine 2,3-dioxygenase (IDO) mRNA expression in response to TLR ligand stimulation. Methods The
expressions of TLRs and IDO mRNA in human HTR-8/SVneo cells were tested by RT-PCR, and the changes in IDO mRNA
levels after exposure to TLR3, TLR4, TLR7/8, and TLR9 ligands were quantitatively analyzed with real-time PCR. Results
IDO and TLR1-10 mRNAs were expressed in HTR-8/SVneo cells. As the cell culture time extended, IDO mRNA expression
level tended to increase within 48 h. After stimulation with the TLR ligands, the expression of TLR-3 mRNA was
down-regulated while the expression of TLR-4, 7, 8, and 9 mRNA up-regulated. Stimulation of the cells with poly(I:C) lowered
the expression of IDO mRNA while IFN-γ increased its expression. Conclusions The expression of IDO mRNA is associated
with the nutrition of the maternal-fetal interface. Stimulation with the TLR ligands affects the expression of IDO and TLR
mRNA expressions in the cells, which verifies the functional activity of TLRs and suggests a role of IDO in TLR
pathway-dependent antiviral immunity.