南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (10): 1474-.

• • 上一篇    下一篇

炎症性肠病患者肠道菌群结构的变化及其与炎性指标的关系

张婷，陈烨，王中秋，周有连，张绍衡，王浦，谢珊，姜泊   

  • 出版日期:2013-10-20 发布日期:2013-10-20

Changes of fecal flora and its correlation with inflammatory indicators in patients with inflammatory bowel disease

  • Online:2013-10-20 Published:2013-10-20

PDF

1193

摘要： 目的探讨炎症性肠病患者肠道菌群结构变化及其与发病的关系。方法收集167例炎症性肠病患者［其中溃疡性结肠炎
（UC）113例，克罗恩病（CD）54例］、54例健康志愿者新鲜粪便，用梯度稀释法定量培养进行菌群分析，同时收集白细胞、血小板、
C反应蛋白、血沉四项炎性指标数据评价其与菌群变化的相关性。结果UC患者与健康对照组相比，肠球菌（6.60±0.23，P<
0.01）、酵母菌（2.22±0.27，P<0.05）、拟杆菌（5.57±0.28，P<0.001）、双歧杆菌（5.08±0.30，P<0.01）、消化球菌（6.22±0.25，P<0.001）、
乳酸杆菌（6.00±0.26，P<0.001）、小梭菌（3.57±0.30，P<0.05）的数量显著增加，真杆菌（1.56±0.24，P<0.01）的数量显著下降。CD
患者与健康对照组相比，肠球菌（6.93±0.28，P<0.01）、酵母菌（2.73±0.37，P<0.01）、拟杆菌（4.32±0.52，P<0.05）、双歧杆菌（4.88±
0.42，P<0.05）、消化球菌（6.19±0.32，P<0.01）、乳酸杆菌（4.73±0.47，P<0.001）的数量显著增加，真杆菌（1.01±0.29，P<0.01）、小梭
菌（0.87±0.31，P<0.01）的数量显著下降。培养阳性率与其数量变化结果基本符合。炎性指标与菌群相关不大。结论与健康对
照组相比，IBD患者肠道菌群呈失衡状态。真杆菌减少与炎症性肠病相关。

Abstract: Objective To investigate the changes in fecal flora and its correlation with the occurrence and progression of
inflammatory bowel disease (IBD). Methods We collected fresh fecal specimens from 167 IBD patients (including 113 with
ulcerative colitis and 54 with Crohn’s disease) and 54 healthy volunteers. The fecal flora was analyzed by gradient dilution
method and the data of inflammatory markers including WBC, PLT, CRP and ESR were collected to assess the association
between the fecal flora and the inflammatory markers. Results The species Enterrococcus (6.60 ± 0.23, P<0.01), Saccharomyces
(2.22 ± 0.27, P<0.05), Bacteriodes (5.57 ± 0.28, P<0.001), Bifidobacterium (5.08 ± 0.30, P<0.01), Peptococcus (6.22 ± 0.25, P<0.001),
Lactobacillus (6.00±0.26, P<0.001), and Clostridium (3.57±0.30, P<0.05) all increased significantly, while Eubacterium (1.56±0.24, P<
0.01) reduced markedly in patients with ulcerative colitis compared with those in the control subjects. Enterrococcus (6.93±0.28,
P<0.01), Saccharomyces (2.73±0.37, P<0.01), Bacteriodes (4.32±0.52, P<0.05), Bifidobacterium (4.88±0.42, P<0.05), Peptococcus (6.19±
0.32, P<0.01) and Lactobacillus (4.73±0.47, P<0.001) all increased significantly and Eubacterium (1.01±0.29, P<0.01) and Clostridium
(0.87±0.31, P<0.01) decreased in patients with Crohn’s disease. The positivity rates of bacterial culture were consistent with the
results of quantitative analysis of the fecal flora. The changes in fecal flora did not show a significant correlation with these
inflammatory markers. Conclusion IBD patients have fecal flora imbalance compared with the healthy controls, and this
imbalance may contribute to the occurrence and progression of IBD. The decline of Eubacterium contributes to the occurrence
and development of IBD.