南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (10): 1471-.

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小白菊内酯对高糖诱导的大鼠肾小球系膜细胞增殖及核因子-κB、单核细胞趋化蛋白-1表达的影响

许兆忠，贾倩倩，李红瑜，王建成，赵艳，陈斯佳，龙海波   

  • 出版日期:2013-10-20 发布日期:2013-10-20

Effects of parthenolide on high glucose-induced cell proliferation, NF-κB activation and MCP-1 expression in rat mesangial cells

  • Online:2013-10-20 Published:2013-10-20

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摘要： 目的探讨小白菊内酯（PTL）对高糖诱导的大鼠肾小球系膜细胞（MC）增殖、核因子-κB（NF-κB）活性和单核细胞趋化蛋
白-1（MCP-1）表达的影响。方法采用正常葡萄糖组（糖浓度为5.6 mmol/L）和高糖组（糖浓度为30 mmol/L）、高糖+PTL组，分
别体外培养大鼠肾小球MC，MTT法检测MC的增殖，ELISA法检测培养液上清中MCP-1的浓度，Western Blot法检测IκBα反
映NF-κB的活性，EMSA法检测NF-κB的活性。结果高糖诱导后MC增殖、MCP-1表达和NF-κB活性明显增强，NF-κB结合蛋
白IκBα明显降解；应用PTL干预后，MC增殖、MCP-1表达和NF-κB活性均明显降低，IκBα降解被抑制。结论PTL能抑制高糖
诱导的肾小球MC NF-κB活化及MCP-1的表达，从而为PTL用于临床防治糖尿病肾病提供了一定的理论依据。

Abstract: Objective To explore the effects of parthenolide (PTL) on high glucose-stimulated cell proliferation, NF-κB activation
and monocyte chemoattractant protein-1 (MCP-1) expression in rat mesangial cells (MCs). Methods MCs were cultured in
normal glucose (5.6 mmol/L), high glucose (30 mmol/L), or high glucose with PTL. MTT assay was used to detect the cell
proliferation. MCP-1 content in the supernatant was measured by ELISA, and the level of IκBα was detected by Western
blotting to reflect NF-κB activity. EMSA method was used to measure the activation of NF-κB. Results MC proliferation,
MCP-1 expression and NF-κB activation were significantly enhanced and the expression of NF-κB-binding protein IκBα was
obviously reduced in cells cultured in high glucose. Application of PTL obviously abolished the effects of high glucose.
Conclusion PTL can suppress high glucose-stimulated NF-κB activation and MCP-1 expression in rat MC. These data provide
a theoretical basis for the clinical application of PTL in prevention and control of diabetic nephropathy.