南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (10): 1453-.

• • 上一篇    下一篇

白芍、赤芍化学成分差异及其对大鼠胸动脉平滑肌A7r5细胞的生物效应比较

朱雅玲，周若龙，吴金雄，张娴，杨柳   

  • 出版日期:2013-10-20 发布日期:2013-10-20

Comparison of chemical components between Baishao and Chishao water extracts and their effects on proliferation of rat thoracic aorta smooth muscle cells

  • Online:2013-10-20 Published:2013-10-20

PDF

1697

摘要： 目的比较白芍、赤芍水提物中所含化学成分的异同，及探讨白芍、赤芍水提物作用于体外大鼠胸动脉平滑肌株（A7r5）的
生物效应差异。方法通过HPLC及质谱分析，比较白芍、赤芍水提物中各单体化学成分的共性与差异。采用体外培养A7r5，利
用血小板源生长因子BB（PDGF-BB）诱导A7r5建立细胞增殖病理模型，加入不同剂量的白芍、赤芍水提物，通过MTT比色法和
实时细胞分析仪检测细胞活性。结果白芍、赤芍在化学成分上有差别，即使两者拥有共同的成分，含量亦存在差异。在A7r5
增殖研究显示，300 μg/ml 白芍水提液与A7r5 对照组相比，细胞增殖显著增加（P<0.01）；而赤芍水提液对其增殖并不显著（P>
0.05）。100~500 μg/ml浓度的赤芍水提物与PDGF-BB病理模型组相比，细胞增殖有显著抑制（P<0.01）；而白芍水提液对其增
殖并不明显（P>0.05）。结论白芍、赤芍水提物在化学成分上存在差异，并在细胞活性研究中，两者亦表现出不同的生物效应。

Abstract: Objective To compare the chemical components in Baishao and Chishao water extracts and investigate their effects on
the proliferation of rat thoracic aorta smooth muscle cells in vitro. Methods The contents and chemical structures of monomers
separated from the water extracts of Baishao and Chishao were analyzed using high-performance liquid chromatography and
mass spectroscopy. Rat thoracic aorta smooth muscle cell line A7r5 and its platelet-derived growth factor-BB
(PDGF-BB)-induced proliferation model were exposed to different concentrations of Baishao and Chishao water extracts, and the
cell viability was analyzed by mitochondrial-dependent reduction of MTT and real-time cell analyzer. Results The growth of
A7r5 cells was significantly stimulated by 300 μg/ml Baishao water extract (P<0.01), but Chishao water extract produced no such
effect (P>0.05). In PDGF-BB-induced cell proliferation model, the cell growth was significantly suppressed by 100-500 μg/ml
Chishao water extract (P<0.01), while Baishao water extract showed no obvious effect on the cell proliferation (P>0.05).
Conclusion Baishao and Chishao water extracts have different chemical components and produce different biological effects.