南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (10): 1442-.

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洗心汤对SAD大鼠脑内tau蛋白O-GlcNAc 糖基化修饰相关酶的影响

第五永长，田金洲，时晶   

  • 出版日期:2013-10-20 发布日期:2013-10-20

Effects of Xixin decoction on enzymes related to O-GlcNAc glycosylation of tau proteins in the brain of rats with sporadic Alzheimer’s disease

  • Online:2013-10-20 Published:2013-10-20

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摘要： 目的建立SAD动物模型，研究名方洗心汤对SAD模型大鼠脑组织tau蛋白O-GlcNAc 糖基化修饰中蛋白质O位N-乙酰
葡萄糖胺糖基转移酶（OGT）、和糖苷酶（O-GlcNAcase）的影响，探讨名方洗心汤防治SAD的可能作用机制。方法将SPF级健
康雄性SD大鼠随机分为假手术组、模型组、多奈哌齐对照组、名方洗心汤小、中、大剂量组。治疗结束及行为学测试之后，以免
疫组化及蛋白印迹方法检测大鼠脑组织tau蛋白O- GlcNAc 糖基化修饰过程中OGT、O-GlcNAcase酶表达的变化。结果名方
洗心汤能显著提高SAD大鼠海马OGT的表达，降低O-GlcNAcase的表达，组间差异有统计学意义（P<0.05，P<0.01）。多奈哌齐
对照组与SAD模型组相比无显著性差异（P>0.05）。结论名方洗心汤能够调节O-GlcNAc 糖基转移酶和糖苷酶的表达，从而提
高SAD大鼠海马组织tau蛋白O-GlcNAc 糖基化修饰水平。

Abstract: Objective: To study the effects of Xixin decoction (XXD) on O-GlcNAc transferase (OGT) and O-GlcNAc glycosidase
in O-GlcNAc glycosylation of tau proteins in the brain of rats with sporadic Alzheimer’s disease (SAD) and explore the
possible mechanism. Methods Male SD rats were randomly divided into sham-operated group, model group, donepezil
group, and low-, moderate-, and high-dose XXD groups. After treatment and behavioral test, the rats were sacrificed for
detecting the expressions of OGT and O-GlcNAc glycosidase in the brain using immunohistochemistry and Western blotting.
Results XXD significantly enhanced the expressions of OGT in the hippocampus of SAD rats and lowered the expression of
O-GlcNAc glycosidase (P<0.05 or 0.01). OGT and O-GlcNAc glycosidase expressions showed no significant differences
between the model group and donepezil group (P>0.05). Conclusion XXD can regulate the expression of OGT and O-GlcNAc
glycosidase to enhance O-GlcNAc glycosylation of tau proteins in the hippocampus of SAD rats.