南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (09): 1367-.

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短肽RGDSY-CTTHWGFTLC对乳腺癌细胞转移和增殖的影响

黄榕权，龙捷，张惠球，张雅洁   

  • 出版日期:2013-09-20 发布日期:2013-09-20

The synthetic peptide RGDSY-CTTHWGFTLC inhibits metastasis and proliferation of
breast cancer cells in vitro

  • Online:2013-09-20 Published:2013-09-20

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摘要： 目的研究短肽RGDSY-CTTHWGFTLC对乳腺癌MCF-7细胞的抗肿瘤能力，为开发更高效的肽类抗肿瘤药物奠定实验
理论基础。方法（1）化学合成目标短肽RGDSY-CTTHWGFTLC（简称RGDSY-CTT），阳性对照肽CTTHWGFTLC（简称
CTT），阴性对照肽STTHWGFTLS（简称STT）；（2）将MCF-7 细胞接种于预涂布纤连蛋白的96 孔板，与短肽共孵育2 h，检测
RGDSY-CTT对MCF-7细胞粘附能力的影响；（3）将MCF-7细胞接种于预铺基质胶的Transwell小室，与短肽共孵育16 h，检测
RGDSY-CTT对MCF-7细胞侵袭能力的影响；（4）与短肽共孵育12 h后，MTT法检测RGDSY-CTT对MCF-7细胞增殖能力的影
响；（5）与短肽共孵育24 h后，PI染色流式法检测RGDSY-CTT对MCF-7细胞凋亡和细胞周期的影响。结果（1）短肽对MCF-7
细胞粘附能力的影响：RGDSY-CTT在终浓度为50、100、200 μg/ml时，MCF-7细胞的粘附率依次为85.1%、74.1%和63.8%，随着
浓度升高，粘附率明显降低，且比CTT组下降更显著（P<0.05）；（2）短肽对MCF-7细胞侵袭能力的影响：RGDSY-CTT在终浓度
为100、200 μg/ml时，对MCF-7细胞的侵袭抑制率分别为41.8%和63.9%；两个浓度均有明显抑制（P<0.01）；但与CTT组比抑制
无显著差异（P>0.05）；（3）短肽对MCF-7细胞增殖的影响：RGDSY-CTT在终浓度为50、100、200 μg/ml时，MCF-7细胞的增殖率
依次为98.8%、82.4%和63.0%，随着肽浓度升高，增殖率下降明显；在100、200 μg/ml时增殖抑制能力比CTT更强（P<0.05）；（4）
短肽对MCF-7细胞凋亡和细胞周期的影响：RGDSY-CTT处理组MCF-7细胞经流式法检测到凋亡峰，细胞周期阻滞发生在G0/
G1期，占76.7%，显著高于CTT组、STT组及正常对照组（P<0.01）。结论RGDSY-CTT能抑制乳腺癌细胞的侵袭能力，同时还具
有抑制乳腺癌细胞粘附、增殖和诱导肿瘤细胞凋亡的能力。

Abstract: Objective To study the effect of the synthetic peptide RGDSY-CTTHWGFTLC on the biological behavior of breast
cancer MCF-7 cells in vitro. Methods MCF-7 cells were incubated with different concentrations of the synthesized peptide
RGDSY-CTTHWGFTLC (RGDSY-CTT), the positive control peptide CTTHWGFTLC (CTT), or the negative control peptide
STTHWGFTLS (STT) in fibronectin-coated 96-well plates for different time lengths, and the changes in cell adhesion,
invasiveness, proliferation, apoptosis and cell cycle were detected using Transwell chamber assay, MTT assay, and flow
cytometry. Results Incubation of the cells with 50, 100 and 200 μg/ml of RGDSY-CTT caused a significant concentrationdependent
inhibition of the cell adhesion (cell adhesion rates of 85.1%, 74.1% and 63.8%, respectively) with stronger effects
than CTT (P<0.05). At 100 and 200 μg/ml, RGDSY-CTT significantly inhibited the invasion (with inhibition rate of 41.8% and
63.9%, respectively) of MCF-7 cells with an effect similar to that by CTT (P>0.05). At 50, 100 and 200 μg/ml, RGDSY-CTT
concentration-dependently suppressed MCF-7 cell proliferation (with cell proliferation rates of 98.8% , 82.4% and 63.0% ,
respectively), and this inhibitory effect was stronger than that of CTT at 100 and 200 μg/ml (P<0.05). The results of flow
cytometry also demonstrated a stronger apoptosis-inducing effect of RGDSY-CTT (76.7%) than that in CTT, STT and the blank
control groups (P<0.05). Conclusions RGDSY-CTT can inhibit cell invasion, suppress adhesion and proliferation, and induce
apoptosis in MCF-7 cells.