南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (09): 1357-.

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线粒体呼吸链复合物Ⅰ缺陷与海洛因海绵状白质脑病的关系

周亮，林敏仕，尹恝   

  • 出版日期:2013-09-20 发布日期:2013-09-20

Relationship between heroin spongiform leucoencephalopathy and respiratory chain complex I deficiency

  • Online:2013-09-20 Published:2013-09-20

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摘要： 目的探讨线粒体呼吸链复合物Ⅰ缺陷与海洛因海绵状白质脑病的关系。方法采用酶联免疫分析方法，应用双抗体夹
心，即线粒体呼吸链复合物Ⅰ抗体-线粒体呼吸链复合物Ⅰ抗原-酶标抗体复合物，加底物TMB显色，用酶标仪在450 nm波长下
测定吸光度。采用上述方法分别测定36例急性期海洛因海绵状白质脑病患者与36例正常对照者外周血白细胞线粒体呼吸链
复合物Ⅰ活性的吸光度，并比对标准曲线，计算样品中人线粒体呼吸链复合物I浓度。结果正常对照组和病患组线粒体呼吸链
复合物Ⅰ活性测定结果分别为5.6±2.4、4.2±2.1 U/ml，两组间有显著差异（t=2.634，P<0.05）。结论急性期海洛因海绵状白质脑
病患者线粒体呼吸链复合物Ⅰ活性降低，存在线粒体功能缺陷。推测脑白质的广泛脱髓鞘与能量代谢障碍有关，线粒体功能障
碍在海洛因海绵状白质脑病发病过程中可能起到重要作用。

Abstract: Objective To investigate the relationship between heroin spongiform leucoencephalopathy and respiratory chain
complex I deficiency. Methods The activity of respiratory chain complex I in peripheral white blood cell mitochondria was
compared between 36 cases of heroin spongiform leucoencephalopathy and 36 healthy subjects using enzyme-linked
immunosorbent assay (ELISA). Results The activity of respiratory chain complex I was 5.6±2.4 U/ml in patients with heroin
spongiform leucoencephalopathy, signficiantly higher than that in the normal subjects (4.2 ± 2.1 U/ml, t=2.634, P<0.05).
Conclusion In patients with heroin spongiform leucoencephalopathy, mitochondrial dysfunction results in energy metabolism
disorder to cause extensive demyelination of the cerebral white matter. Respiratory chain complex I deficiency of the
mitochondria plays a significant role in the pathogenesis of heroin spongiform leucoencephalopathy.