南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (09): 1332-.

• • 上一篇    下一篇

促红细胞生成素对衰老大鼠脑组织中抗氧化酶活性的影响

翟跃芬，王慧云，王虎清，孙宏，张桂莲，吴海琴   

  • 出版日期:2013-09-20 发布日期:2013-09-20

Effect of erythropoietin on activities of antioxidant enzymes in the brain tissue of aged rats

  • Online:2013-09-20 Published:2013-09-20

PDF

1438

摘要： 目的研究促红细胞生成素（EPO）对衰老大鼠脑组织中抗氧化酶活性的影响。方法将大鼠随机分为3组：正常对照组、衰
老模型组及重组人促红细胞生成素（rhEPO）干预组，每组10只。行为学实验后处死大鼠，行尼氏染色在光镜下观察海马组织神
经元内的尼氏体数量；同时检测脑组织中过氧化氢酶（CAT）及谷胱甘肽过氧化物酶（GSH-Px）的活性。结果①衰老组较对照
组大鼠学习记忆能力下降（P<0.05），干预组较衰老组大鼠的学习记忆能力改善（P<0.05）；②光镜下见衰老组大鼠神经元内尼氏
体数量较对照组明显减少，干预组较衰老组明显增多，但少于对照组；③衰老组大鼠脑组织中的CAT、GSH-Px活性均较对照组
显著降低（P<0.05），而干预组的CAT、GSH-Px活性较衰老模型组显著升高（P<0.05）。结论EPO可增强衰老大鼠脑组织中抗氧
化酶的活性，提高其抗氧化能力从而起到抗衰老作用。

Abstract: Objective To study the effect of erythropoietin (EPO) on the activities of antioxidant enzymes, namely catalase (CAT)
and glutathione peroxidase (GSH-Px) in the brain tissues of aged rats. Methods Thirty SD rats were randomly divided into
normal control, aging model, and recombinant human erthropoietin (rhEPO) treatment groups (n=10). Morris water maze was
used to compare the behavioral indexes. The rats were then sacrificed to observe Nissl bodies in the hippocampal neurons
with Nissl staining and test the activities of CAT and GSH-Px in the brain tissues. Results Compared with the control group,
the aging rats showed significantly deteriorated learning and memory abilities (P<0.05), which were improved obviously by
rhEPO treatment (P<0.05). The number of Nissl bodies in the neurons was reduced in the aging rats compared with that in the
control group, and rhEPO treatment increased the number of Nissle bodies but failed to restore the control level. The aging
rats also showed significantly lowered activities of CAT and GSH-Px in the brain tissue (P<0.05), which were increased
significantly after rhEPO treatment (P<0.05). Conclusion EPO can enhance the activities of the antioxidant enzymes in the
brain tissues of aged rats to increase the antioxidant capacity and produces an anti-aging effect.