南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (09): 1329-.

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水通道蛋白基因4在子痫前期模型大鼠脑组织中的表达

刘磊，刘慧姝，黄倩，高旖鑫，胡碧辉   

  • 出版日期:2013-09-20 发布日期:2013-09-20

Expression of aquaporin-4 protein in the brain of preeclampsia model rats

  • Online:2013-09-20 Published:2013-09-20

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摘要： 目的研究水通道蛋白基因4（AQP4）在子痫前期模型鼠脑组织中的表达变化。方法成年SD大鼠分为PE模型组（7只）通
过尾静脉注射内毒素（1 μg/kg）的方法建立，正常妊娠组（6 只）和非孕组（12 只）注射等量生理盐水。应用免疫组化和
Western-blot检测各组大鼠海马区AQP4的表达水平。结果妊娠第19日PE模型组大鼠血压（135±9）mmHg明显高于正常妊娠
组（116±8）mmHg和非孕组（112±6）mmHg（P<0.02）；PE模型组尿蛋白（3.8±0.5）水平明显高于正常妊娠组（2.6±0.6）和非孕组
（2.3±0.4）（P<0.05）。免疫组化结果显示AQP4 集中分布在脑内血管壁，Western-blot 灰度分析提示子痫前期大鼠海马组织中
AQP4蛋白的表达水平（41.7±4.2）明显高于正常妊娠组（26.3±5.3）和非孕组（9.2±2.1）（P<0.01）。结论AQP4在子痫前期模型大
鼠脑组织中表达上调，可能与子痫发病有关。

Abstract: Objective To study the expression of aquaporin-4 (AQP4) protein in preeclampsia(PE) model rats. Methods Adult
SD rats were divided into PE model group (n=7) induced by endotoxin, normal pregnancy group (n=6) and non-pregnancy
group (n=12) treated with an equal volume of saline. AQP4 protein expression in the brain of the rats was detected with
immunohistochemical staining and Western blotting. Results The blood pressure in PE model rats (135 ± 9 mmHg) was
significantly higher than that in normal pregnancy rats (116±8 mmHg) and non-pregnancy rats (112±6 mmHg) (P<0.02). The
rats in PE model group showed obvious proteinuria compared with the other two groups (3.8±0.5 vs 2.6±0.6 and 2.3±0.4, P<
0.05). Immunohistochemistry showed that AQP4 protein was localized primarily around the brain parenchymal blood vessels.
Western analysis revealed a significantly elevated AQP4 protein expression in PE model group (39.6±4.9) compared with that
in normal pregnancy group (26.5±4.3) and non-pregnancy group (9.7±2.1) (P<0.01). Conclusion The up-regulation of AQP4
protein around the intraparenchymal blood vessels of the brain may play a role in the development of eclampsia.