南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (09): 1325-.

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miR-19a对溃疡性结肠炎的作用机制

周鹏志，陈斌，胡品津，孙嫣   

  • 出版日期:2013-09-20 发布日期:2013-09-20

Role of miR-19a in ulcerative colitis in mice

  • Online:2013-09-20 Published:2013-09-20

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摘要： 目的探讨miR-19a对溃疡性结肠炎的作用机制。方法通过生物信息学分析预测miR-19a可能的靶基因，通过免疫组化
及Western blotting技术检测靶基因在溃疡性结肠炎小鼠中表达的变化，并进一步通过绿色荧光蛋白报告载体实验对靶基因进
行鉴定。结果生物信息学分析预测miR-19a可能的靶基因是TNF-α，免疫组化及Western blotting显示溃疡性结肠炎小鼠肠道
组织TNF-α表达增加，miR-19a 可抑制TNF-α-3’UTR-WT报告基因活性，而变异型TNF-α-3’UTR-mut 报告基因活性不能被抑
制。结论miR-19a的靶基因为TNF-α，其结合位点为TNF-α 3’UTR，miR-19a可能在肠道通过直接调控TNF-α而发挥作用。

Abstract: Objective To study the role of miR-19a in ulcerative colitis (UC) in mice. Methods The target gene of miR-19a was
predicted by bioinformatics analysis. The expression of the target protein in UC colon was detected by immunohistochemistry
and Western blotting. The target gene was further identified by enhanced green fluorescent protein (EGFP) report vector
system. Results The target gene of miR-19a was TNF-α as predicted by bioinformatics analysis. TNF-α expression was highly
expressed in the colonic tissue of UC mice. MiR-19a could inhibit the report gene activity of TNF-α-3’UTR-WT but no that of
TNF-α-3’UTR-Mut. Conclusion The target gene of miR-19a is TNF-α, and the binding site is TNF-α 3’UTR. The possible role of
miR-19a in UC pathogenesis involves regulation of TNF-α expression in the colon.