南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (08): 1137-.

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芹菜素对人肺癌NCI-H460细胞增殖及凋亡的影响

潘雪刁，杨周萍，杨志英，周四桂，张海涛，臧林泉   

  • 出版日期:2013-08-20 发布日期:2013-08-20

Effect of apigenin on proliferation and apoptosis of human lung cancer NCI-H460 cells

  • Online:2013-08-20 Published:2013-08-20

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摘要： 目的研究芹菜素对人肺癌NCI-H460 细胞增殖及凋亡的影响。方法应用10~80 μmol/L 芹菜素处理NCI-H460 细胞，
MTT法检测细胞增殖抑制率；Hoechst 33258细胞核染色法观察细胞凋亡的形态学改变；流式细胞仪AnnexinV-FITC/PI双染色
法检测细胞凋亡率；Western blotting检测凋亡相关信号分子Bax、Bcl-2和caspase-3的蛋白表达水平。结果芹菜素对NCI-H460
细胞的增殖抑制作用呈浓度和时间依赖性；芹菜素处理组细胞出现核固缩、染色质凝集和核碎片化等典型的细胞凋亡特征；
NCI-H460细胞凋亡率随着芹菜素浓度增加逐渐增高；芹菜素处理组细胞Bax和caspase-3蛋白表达增加，Bcl-2蛋白表达降低。
结论芹菜素能够抑制人肺癌NCI-H460细胞的增殖，并诱导其凋亡，其机制可能与上调Bax和下调Bcl-2的表达，导致Bcl-2/Bax
比值下降，caspase-3活化有关。

Abstract: Objective To study the effect of apigenin on the proliferation and apoptosis of human lung cancer cell line
NCI-H460. Methods NCI-H460 cells were cultured with different concentrations of apigenin, and MTT assay was used to
evaluate the cell inhibition rates. Apoptosis of NCI-H460 cells was observed under a fluorescence microscope with Hoechst
33258 staining and quantified by flow cytometry using annexin V-FITC/PI stain. The expressions of apoptosis-related proteins
Bax, Bcl-2 and caspase-3 were analyzed by Western blotting. Results Apigenin causes concentration- and time-dependent
inhibition of the proliferation of the cells. NCI-H460 cells treated with apigenin showed significant morphological changes of
apoptosis, and the cell apoptotic rates increased as apigenin concentration increased. Western blotting demonstrated that
apigenin increased the protein levels of Bax and caspase-3 and reduced the protein expression of Bcl-2. Conclusion Apigenin
can inhibit the proliferation and induce apoptosis of NCI-H460 cells possibly by up-regulating expression of Bax and caspase-3
and down-regulating the expression of Bcl-2.