南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (08): 1127-.

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MicroRNA-218在肝细胞癌中的表达及功能

李超，涂康生，郑鑫，张竞，拓航，高洁，姚英民，刘青光   

  • 出版日期:2013-08-20 发布日期:2013-08-20

MicroRNA-218 expression and its role in hepatocellular carcinoma

  • Online:2013-08-20 Published:2013-08-20

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摘要： 目的检测MicroRNA-218（miR-218）在肝细胞癌中的表达情况并研究其在肝癌中的功能。方法收集46例肝细胞癌及对
应癌旁组织，运用qRT-PCR技术检测miR-218 在肝癌及对应癌旁组织中的表达情况；应用miR-218 mimic转染人肝癌细胞系
HepG2，MTT和流式细胞术检测转染后细胞活力和凋亡变化，qRT-PCR和Western blot检测miR-218潜在靶点的表达变化。结
果miR-218在肝癌组织中的表达显著低于对应的癌旁组织（P<0.05）；miR-218低表达与肿瘤大小（>5 cm）和高TNM分期（Ⅲ+
Ⅳ）具有显著相关性（P<0.05）；在HepG2细胞中，miR-218抑制细胞增殖和诱导凋亡，并下调Bmi-1和CDK6 mRNA及蛋白表达
（P<0.05）。结论miR-218低表达与肝癌的恶性临床病理特征相关，miR-218可能通过下调Bmi-1和CDK6表达抑制肝癌细胞增
殖和促进凋亡。

Abstract: Objective To investigate the expression of microRNA-218 (miR-218) and its role in hepatocellular carcinoma (HCC).
Methods Forty-six pairs of fresh surgical specimens of HCC and adjacent tissues were examined for miR-218 expression using
qRT-PCR. A miR-218 mimic was transfected into HepG2 cells, and the cell viability and apoptosis were analyzed by MTT assay
and flow cytometry, and the potential targets of miR-218 were detected by qRT-PCR and Western blotting. Results The
expressions of miR-218 in HCC tissues were significantly down-regulated compared to those in the adjacent tissues (P<0.05).
Down-regulation of miR-218 was found to correlate significantly with the tumor size (>5 cm) and an advanced TNM stage (III+
IV) (P<0.05). Ectopic expression of miR-218 in HepG2 cells resulted in suppressed cell proliferation and enhanced cell
apoptosis as well as the down-regulation of Bmi-1 and CDK6 mRNA and protein expressions (P<0.05). Conclusion The
low-expression of miR-218 is correlated with malignant clinicopathological characteristics of HCC, and miR-218 may inhibit
cell proliferation and promote cell apoptosis by down-regulating Bmi-1 and CDK6 in HCC.