南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (08): 1099-.

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Voluntary exercise promotes proliferation and differentiation of adult rat hypothalamus progenitor cells

李光华，松崎健太郎，王银，赵楠，杨敏，紫藤治   

  • 出版日期:2013-08-20 发布日期:2013-08-20

自主运动可促进大鼠下丘脑神经祖细胞的增殖与分化

  • Online:2013-08-20 Published:2013-08-20

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摘要： 目的探讨自主运动对大鼠下丘脑神经细胞增殖与分化的影响。方法雄性Wistar大鼠，随机分为对照组（SE）和自主运动
组（EX），以上两组又进一步分为6、13、23、33、43、53 d共6个亚组。各组大鼠从实验开始，连续5 d腹腔注射BrdU，于上述时间
点处死大鼠并取脑组织观察下丘脑的BrdU阳性细胞数的变化。结果免疫组化结果表明，EX组下丘脑的BrdU阳性细胞数在
23、33、43、53 d亚组中明显高于SE组（P<0.05）。EX组中经43 d的自主运动后，BrdU阳性细胞数与神经元双染色细胞数明显增
加（P<0.05），对照组各亚组则无显著性变化。结论自主运动可促进下丘脑的神经祖细胞增殖并分化为神经元，这可能与长期
的运动锻炼有一定关系。

Abstract: Objective To investigate the effect of voluntary exercise on the proliferation and differentiation of hypothalamus
progenitor cells in adult rats. Methods Male Wistar rats were divided into voluntary exercise (EX) and sedentary (SE) groups,
both of which were further divided into 6 subgroups for observation on days 6, 13, 23, 33, 43 and 53. Bromodeoxyuridine
(BrdU) was intraperitoneally injected daily for 5 consecutive days after commencing voluntary exercise, and at the specified
time points during voluntary exercise, the rats’ brains were removed to observe the numbers of BrdU-positive cells in the
hypothalamus. Results Immunohistochemical analysis showed that the numbers of BrdU-positive cells in the hypothalamus of
EX subgroups were significantly greater than those of SE subgroups on days 23, 33, 43 and 53. In EX group, the number of
BrdU-positive cells double-stained for a mature neuron marker increased after 43 days of voluntary exercise, which did not
occur in SE group. Conclusion Long-term voluntary exercise can promote the proliferation of neuronal progenitor cells in the
hypothalamus and their differentiation into neurons.