南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (07): 994-.

• • 上一篇    下一篇

CXCR7-shRNA慢病毒载体对人肝癌细胞生长及侵袭能力的抑制作用

戴小珍，熊新，王兰，潘克俭，何浪，李红   

  • 出版日期:2013-07-20 发布日期:2013-07-20

Effects of CXCR7-shRNA lentiviral vector on the growth and invasiveness of human hepatoma carcinoma cells in vitro

  • Online:2013-07-20 Published:2013-07-20

PDF

1506

摘要： 目的探讨CXCR7-shRNA慢病毒表达载体靶向抑制CXCR7的表达对人肝癌细胞增殖及侵袭能力的影响。方法利用
RNA干扰技术，构建CXCR7的shRNA慢病毒表达载体，转染人肺转移肝癌细胞株HCCLM3，分别通过RT-PCR和Western blot
检测HCCLM3细胞中CXCR7 mRNA和蛋白质表达水平的变化；然后通过MTT法考察CXCR7沉默对HCCLM3细胞增殖侵袭
能力的影响，通过体外粘附实验和Transwell小室法分别考察CXCR7沉默对HCCLM3细胞粘附及侵袭能力的影响。结果与空
白对照组比较，转染CXCR7-shRNA慢病毒载体的HCCLM3细胞中CXCR7 的mRNA及蛋白水平表达均明显降低（P<0.01），
SDF-1诱导的HCCLM3细胞的增殖能力显著下降（P<0.05），同时HCCLM3细胞的粘附及侵袭能力也明显受到CXCR7-shRNA
的抑制（P<0.01）。结论靶向沉默CXCR7能显著抑制结肝癌细胞的增殖和侵袭能力，为肝癌的治疗提供一个潜在的作用靶点。

Abstract: Objective To explore the effects of CXCR7 knock-down by CXCR7-shRNA lentiviral vector on the proliferation and
invasion of human hepatoma carcinoma cells in vitro. Methods CXCR7-shRNA lentiviral vector was transfected into
heptatocellular carcinoma HCCLM3 cells. The changes in mRNA and protein expression of CXCR7 in the transfected cells
were investigated using real-time PCR and Western blotting, respectively, and MTT assay was employed to assess the cell
proliferation changes. In vitro adhesion assay and transwell chamber test were used to observe the adhesion and invasiveness
of HCCLM3 cells, respectively. Results Transfection of HCCLM3 cells with CXCR7-shRNA lentiviral vector resulted in a
significantly decreased expression of CXCR7 at both mRNA and protein levels (P<0.01) and obvious suppression of the cell
proliferative activity (P<0.05). CXCR7-shRNA also significantly suppressed the invasiveness and adhesion of HCCLM3 cells (P<
0.01). Conclusion CXCR7 knock-down can significantly inhibit the proliferation and invasiveness of human hepatoma
carcinoma cells in vitro, suggesting the value of CXCR7 as a potential target for hepatoma carcinoma therapy.