南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (07): 990-.

• • 上一篇    下一篇

胃泌素对大鼠小肠上皮1,25(OH)2D3膜相关快速反应类固醇结合蛋白的影响

梁芬芬，刘翠平，黎丽旋，郭瑜，白岚   

  • 出版日期:2013-07-20 发布日期:2013-07-20

Effects of gastrin on rat intestinal epithelial 1,25(OH)2D3-membrane associated rapid response steroid binding protein

  • Online:2013-07-20 Published:2013-07-20

PDF

1245

摘要： 目的探讨胃泌素对大鼠小肠上皮1,25(OH)2D3膜相关快速反应类固醇结合蛋白（1,25D3-MARRS结合蛋白）表达的影响。
方法应用免疫组织化学、RT-PCR和免疫印迹法，检测胃泌素、奥美拉唑处理后的SD大鼠小肠中1,25D3-MARRS结合蛋白的表
达情况与对照组的差异，并测大鼠血清中钙、磷离子的浓度变化。结果大鼠小肠上皮细胞中1,25D3-MARRS结合蛋白分布于
胞核、胞浆和胞膜。胃泌素、奥美拉唑均引起大鼠小肠组织1,25D3-MARRS结合蛋白的mRNA和蛋白两个水平表达增高（P<
0.05），但其作为一个促小肠钙磷吸收的蛋白，无明显增高血清钙磷浓度的作用（P>0.05）。结论1,25D3-MARRS结合蛋白作为
一个广泛分布的多功能蛋白，受胃泌素的调节，在消化道相关疾病具有很高的研究价值及临床应用前景。

Abstract: Objective To explore the effects of gastrin on the expression of 1,25(OH)2D3-membrane associated rapid response
steroid (1,25D3-MARRS) binding protein in rat intestinal epithelium. Methods SD rats received intraperitoneal injections of
gastrin, omeprazole or physiological saline. The protein expression of 1,25D3-MARRS binding protein in SD rat intestinal was
determined with Western blotting and immunohistochemistry, and its mRNA levels determined by RT-PCR. The serum
calcium and phosphate levels in the rats were also detected. Results Immunohistochemistry showed that 1,25D3-MARRS
binding protein was expressed mainly in the nuclei, cytoplasm and membrane of the intestinal epithelial cells. Both the protein
and mRNA expression levels of 1,25D3-MARRS binding protein were up-regulated after treatments with gastrin and
omeprazole (P<0.05), but the serum calcium and phosphate concentrations showed no obvious increase. Conclusion 1,
25D3-MARRS binding protein, which is widely expressed with versatile functionalities, is regulated by gastrin and shows high
potentials in the study of gastrointestinal diseases.