南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (07): 962-.

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红景天苷通过抑制凋亡相关蛋白的表达保护缺氧对培养神经干细胞的损伤

  

  • 出版日期:2013-07-20 发布日期:2013-07-20

Salidroside protects cultured rat subventricular zone neural stem cells against hypoxia injury by inhibiting Bax, Bcl-2 and caspase-3 expressions

  • Online:2013-07-20 Published:2013-07-20

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摘要： 目的观察红景天苷对缺氧损伤成年大鼠侧脑室室管膜下区（SVZ）组织分离培养神经干细胞凋亡比例和Bax、Bcl-2、
caspase-3蛋白表达的影响。方法原代培养成年大鼠SVZ神经干细胞，不同浓度红景天苷预处理24 h 后，干细胞厌氧工作站缺
氧损伤48 h。四甲基偶氮唑盐（MTT）法检测各组细胞活力；显微镜下观察各组细胞形态；TUNEL染色和流式细胞仪检测细胞
凋亡情况；Western blot 检测细胞内Bax ，Bcl-2 和caspase-3蛋白的表达变化。结果红景天苷预处理可显著改善缺氧损伤后的
细胞生长状态和活力，降低缺氧引起的细胞凋亡率的升高（P<0.05）；拮抗缺氧损伤导致的细胞Bcl-2/Bax 蛋白比例的下降，降低
活化的caspase-3蛋白的表达（P<0.05）。结论红景天苷通过抑制神经干细胞的凋亡，保护缺氧对神经干细胞的损伤，其机制可
能与调节凋亡相关基因Bax、Bcl-2和caspases-3的表达有关。

Abstract: Objective To explore the effects of salidroside (sal) on the expressions of Bcl-2, Bax and caspases-3 proteins in
cultured rat subventricular zone (SVZ) neural stem cells (NSCs) exposed to hypoxia injury. Methods Primarily cultured SVZ
NSCs from adult SD rats were incubated with salidroside (120 and 240 μmol /L) for 24 h prior to exposure to hypoxia. The cell
viability was assessed with MTT assay, and the cell apoptosis was analyzed using TUNEL staining and flow cytometry. Western
blotting was performed to detect the expressions of Bcl-2, Bax and caspase-3 in the cells. Results Salidroside pretreatment of the
cells for 24 h resulted in an obvious resistance to hypoxia-induced cell apoptosis and decrement of cell viability (P<0.05).
Salidroside also antagonized the effect of hypoxia exposure in lowering Bcl-2/Bax ratio apoptosis of rat neural stem cells and
decreased the expression of caspases-3 protein (P<0.05). Conclusion Salidroside can significantly resist hypoxia-induced. The
neuroprotective effect of salidroside may be related to the modulation of expressions of apoptosis-related proteins.