南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (06): 930-.

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Exendin-4对肿瘤坏死因子-α诱导的单核细胞趋化蛋白-1表达的影响

江颖娟，薛耀明，张倩，张艳飞，袁园   

  • 出版日期:2013-06-20 发布日期:2013-06-20

Effect of exendin-4 on monocyte chemoattractant protein-1 expression in cultured rat glomerular mesangial cells induced by tumor necrosis factor-α in vitro

  • Online:2013-06-20 Published:2013-06-20

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摘要： 目的观察exendin-4对大鼠肾小球系膜细胞单核细胞趋化蛋白-1（MCP-1）、纤维连接蛋白（FN）表达的影响。方法试验分
组：对照组、肿瘤坏死因子-α（TNF-α）组（10 ng/ml）、TNF-α（10 ng/ml）+E1 组（1 nmol/L exendin-4）、TNF-α（10 ng/ml）+E5 组
（5 nmol/L exendin-4）、TNF-α（10 ng/ml）+E10组（10 nmol/L exendin-4）。收集24 h细胞，提取RNA，采用实时荧光定量检测细
胞内MCP-1 mRNA的表达；分别在24、48 h 收集各组细胞培养上清液，采用ELISA检测培养细胞上清中MCP-1、FN的浓度。
结果孵育24 h 时，TNF-α组MCP-1 mRNA和细胞培养上清MCP-1、FN表达较对照组明显增加（P<0.01）；与TNF-α组比较，
TNF-α+E5组、TNF-α+E10组细胞内MCP-1 mRNA和培养上清内MCP-1、FN含量均明显降低；孵育48 h时，TNF-α组细胞培养
上清MCP-1、FN表达较对照组明显增加（P<0.01）；与TNF-α组比较，TNF-α+E1组、TNF-α+E5组、TNF-α+E10组细胞培养上清
内MCP-1、FN含量均明显降低；并且随着时间的延长和exendin-4浓度的增加，培养上清内MCP-1、FN含量降低更加明显。结
论Exendin-4可明显抑制TNF-α诱导的系膜细胞MCP-1 mRNA的表达，并以剂量和时间依赖的方式抑制TNF-α诱导的系膜细
胞培养上清内MCP-1、FN的含量，提示exendin-4可能会对肾脏具有一定保护作用。

Abstract: Objective To investigate the effect of exendin-4 on the expression of monocyte chemoattractant protein-1 (MCP-1)
and fibronectin (FN) in rat glomerular mesangial cells in vitro. Methods Rat glomerular mesangial cells were divided into 5
groups, namely control group, tumor necrosis factor-α (TNF-α) group (10 ng/ml), TNF-α (10 ng/ml)+E1 (1 nmol/L exendin-4)
group, TNF-α (10 ng/ml) + E5 (5 nmol/L exendin-4) group, and TNF-α (10 ng/ml) + E10 (10 nmol/L exendin-4) group. After
cultured 24 h or 48 h, RNA were extracted to determine the expression of MCP-1 with real-time PCR, the supernatant were
collected to determine the expression of MCP-1 and FN with ELISA. Results Compared with control group, the cells treated
with TNF-α for 24 h showed significantly increase the expression of MCP-1 and FN (P<0.01), exendin-4 significantly reduced
the expression of MCP-1 and FN in TNF-α+E5 group and TNF-α+E10 group (P<0.05). After 48 h incubation, the expression of
MCP-1 and FN increased significantly in TNF-α group (P<0.01), which was lowered by exendin-4 in TNF-α+E1,TNF-α+E5 and
TNF-α+E10 groups (P<0.05). Conclusion Exendin-4 has an intrinsic capability to concentration- and time-dependently inhibit
TNF-α-induced expression of MCP-1 and FN in rat mesangial cells, suggesting the beneficial effect of exendin-4 in preventing
and treating diabetic nephropathy.