南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (05): 733-.

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NOD/SCID小鼠中A431 侧群与非侧群细胞成瘤组织中EMT相关分子的表达

邵玲巧，王倩倩，郭圆圆，耿松梅   

  • 出版日期:2013-05-20 发布日期:2013-05-20

Expression of epithelial-mesenchymal transition markers in tumor tissues generated by side population and non-side population of A431 cells in NOD/SCID mice

  • Online:2013-05-20 Published:2013-05-20

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摘要： 目的旨在分离A431中侧群（SP）细胞及非侧群（NSP）细胞亚群，比较二者在小鼠皮下成瘤能力差异及组织中上皮间质转
化（EMT）相关分子表达。方法培养A431细胞经荧光染料Hoeehst33342染色后，流式细胞仪分选出SP细胞和NSP细胞，接
种到NOD/SCID 小鼠皮下，观察各自成瘤能力，取瘤体组织免疫组织化学染色检测EMT相关分子E-Cadherin、β-catenin、
Vimentin、AXL和Erbb3的表达。结果A431细胞系中成功分选出SP细胞，小鼠皮下成瘤迅速。在SP细胞小鼠成瘤组织周围
细胞中，E-Cadherin、Erbb3表达降低，β-catenin、Vimentin、AXL表达增高。结论A431细胞中含有SP细胞亚群，该亚群细胞具
有较强的肿瘤形成能力，且显示较强的EMT特性。

Abstract: Objective To isolate the side population (SP) and non-side population (NSP) cells from A431 cells and compare their
difference in tumorigenicity in mice and the expression profiles of epithelial-mesenchymal transition (EMT) markers. Methods
A431 cells stained with Hoechst 33342 were sorted with flow cytometry. The isolated SP cells and NSP cells were inoculated
into NOD/SCID mice and the tumorigenicity of the cells was observed. EMT markers E-cadherin, β-catenin, vimentin, AXL,
and Erbb3 in the tumor tissues were detected by immuohistostaining. Results The tumors generated by SP cells were larger
than those by NSP cells in NOD/SCID mice. Compared with the tumors generated by NSP cells, the cells in the periphery of
tumors generated by SP cells showed up-regulated expressions of AXL, vimentin and β-catenin and down-regulated ERBB3
and E-cadherin. Conclusion The SP cells in A431 cells have a strong tumorigenicity and show more EMT phenotypes in
tissues.