南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (05): 692-.

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长非编码MALAT1基因在人鼻咽癌细胞株的表达及生物学意义

谢林英，胡志燕，王晓燕，李祖国   

  • 出版日期:2013-05-20 发布日期:2013-05-20

Expression of long noncoding RNA MALAT1 gene in human nasopharyngeal carcinoma cell lines and its biological significance

  • Online:2013-05-20 Published:2013-05-20

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摘要： 目的探讨MALAT1在鼻咽癌细胞细胞株中的表达及生物学功能。方法通过Real-time PCR检测MALAT1基因在鼻炎
癌细胞株5-8F、C666-1、CNE-1、CNE-2、HONE-1、6-10B及永生化鼻咽上皮细胞NP69株中的表达；采用RNAi技术和RNA激活
技术，构建MALAT1慢病毒干扰载体和激活载体载体并稳定转染鼻咽癌细胞株CNE-1，采用CCK8、平板克隆、划痕等试验分析
MALAT1基因对CNE-1细胞生物学行为的影响。结果MALAT1在高转移的鼻咽癌细胞株中高表达，在正常鼻咽上皮细胞低
表达；经激活过表达MALAT1 后，CNE-1 细胞的上皮性标志物E-Cadherin 的表达显著下调，间质细胞标志物Vimentin 表达上
调，侵袭转移能力明显增强（P<0.05）。结论MALAT1基因上调能够促进鼻咽癌CNE-1细胞的增殖、侵袭和转移能力。

Abstract: Objective To explore the expression of MALAT 1 in nasopharyngeal carcinoma (NPC) and its biological function.
Method Real-time PCR was used to detect the expression of MALAT1 in 5-8F, C666-1, CNE-1, CNE-2, HONE-1, 6-10B and
NP69 cell lines. CNE-1 cells engineered with MALAT1 RNA interference (RNAi) and RNA activation (RNAa) techniques were
examined for changes in cell proliferation, invasion and metastasis using CCK8 assay, colony formation assay, Transwell in
vitro invasion assay and wound-healing assay ability. Results MALAT1 was highly expressed in 5-8F cells with a high
metastatic potential, and lowly expressed in normal nasopharyngeal epithelium cells. Overexpression of MALAT1 by RNAa
suppressed the expression of E-cadherin, promoted the expression of vimentin and enhanced the proliferation, invasion, and
metastasis of CNE-1 cells. Conclusion MALAT1 can enhance the proliferation, invasion, and metastasis of CNE-1 cells.