南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (05): 678-.

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封闭内源性miR-23a对人胃腺癌细胞系MGC803增殖及侵袭的影响

朱丽华，田家莉，陈力，王梅梅，熊亚南，章广玲，李淑英，袁丽杰   

  • 出版日期:2013-05-20 发布日期:2013-05-20

Effect of blocking endogenous miR-23a on the proliferation and invasion in gastric adenocarcinoma cell line MGC803

  • Online:2013-05-20 Published:2013-05-20

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摘要： 目的设计并合成miR-23a ASO (ASO-23a)，封闭内源性miR-23a的功能后，检测人胃腺癌细胞系MGC803增殖及侵袭能
力的改变。方法首先设计并合成ASO-23a，经脂质体方法转染MGC803 细胞，实时定量PCR 检测转染细胞MGC803 中
miR-23a的表达水平；经MTT实验、平板克隆形成实验、软琼脂克隆形成实验、TUNEL实验及transwell实验，观察细胞内源性
miR-23a功能被封闭后对MGC803细胞增殖、凋亡及侵袭能力的影响。结果实时定量PCR结果表明ASO-23a能够有效封闭细
胞中内源性miR-23a；MTT、平板克隆形成及软琼脂克隆形成实验结果显示，封闭内源性miR-23a后可抑制MGC803细胞活性；
TUNEL实验结果显示，ASO-23a可以促进MGC803细胞凋亡的发生；Transwell实验结果显示，ASO-23a可以抑制MGC803细
胞的侵袭能力。结论设计并合成的ASO-23a在人胃腺癌细胞系MGC803中有效封闭内源性miR-23a，能够抑制细胞活性及侵
袭能力，并能促进细胞凋亡的发生。

Abstract: Objective To investigate the effect of functional blocking of endogenous miR-23a with a specific antisense
oligonucleotide (ASO) on the proliferation and invasiveness of gastric adenocarcinoma cell line MGC803 in vitro. Methods A
specific ASO targeting miR-23a, namely ASO-23a, was transfected into MGC803 cells to block endogenous miR-23a. The
mRNA level of miR-23a in the transfected cells was detected with quantitative real-time PCR. The changes of cell proliferation
following the transfection were detected with MTT assay and colony formation assay, and TUNEL assay and Transwell assay
were employed to evaluate the changes in cell apoptosis and invasiveness, respectively. Results Quantitative real-time PCR
demonstrated efficient functional blocking of endogenous miR-23a in MGC803 cells by ASO-23a. Suppression of miR-23a with
ASO-23a obviously inhibited cell growth, colony formation and invasiveness of MGC803 cells and significantly enhanced the
cell apoptosis. Conclusion ASO-23a can efficiently block the function of endogenous miR-23a in MGC803 cells to inhibit cell
proliferation and invasion and promote cell apoptosis.