南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (05): 667-.

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多发性骨髓瘤细胞中SWI/SNF核心亚单位SNF5调控的基因表达谱分析

谢莹莹，徐旸   

  • 出版日期:2013-05-20 发布日期:2013-05-20

Changes in gene expression profiles in multiple myeloma cells after knocking-down SNF5, a core subunit of SWI/SNF complex

  • Online:2013-05-20 Published:2013-05-20

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摘要： 目的研究染色质重塑复合物SWI/SNF核心亚单位SNF5对骨髓瘤细胞系基因表达谱的调控作用。方法在KM3细胞中
构建四环素诱导的表达SNF5 siRNA的稳定细胞系, 通过四环素诱导敲低SNF5，检测SNF5敲低前后的基因表达谱，对差异表
达基因进行生物信息学分析，并对部分基因进行验证。结果SNF5敲低抑制KM3细胞生长，基因表达谱分析发现545个表达差
异基因，其中214个上调，331个下调。结论SNF5是多发性骨髓瘤细胞生长所需的，它通过影响与细胞生长及凋亡相关基因的
表达来发挥作用。

Abstract: Objective To investigate the changes in the gene expression profiles in a multiple myeloma cell line after
knocking-down the expression of SNF5, a core component of the SWI/SNF chromatin remodeling complex. Methods The total
RNA were extracted from tetracycline-inducible SNF5 knockdown (KD) cell line derived from KM3 cells for gene expression
profiling by affymetrix microarray and bioinformatic analysis. Results Knockdown of SNF5 inhibited KM3 cell proliferation.
A total of 545 genes were found to be differentially expressed in the cells with SNF5 knockdown, among which 214 were
up-regulated and 331 were down-regulated. Conclusions SNF5 is essential for the growth of multiple myeloma cells and can
regulate the expression of the genes associated with cell growth and apoptosis.