南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (04): 559-.

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COX-2、PKC-α和miR-101 在胃癌中的表达及相关性

孙海兵，魏永长，涂宏蕾，杜宁，赵阳，胡丽娟，任宏   

  • 出版日期:2013-04-20 发布日期:2013-04-20

  • Online:2013-04-20 Published:2013-04-20

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摘要： 目的探讨miR-101、蛋白激酶C-α（PKC-α）和环氧合酶2（COX-2）在胃癌中的表达及相关性。方法（1）荧光定量RT-PCR
法检测57例胃癌组织标本对应的正常胃粘膜标本中miR-101的表达水平；（2）Western blotting检测57例胃癌组织标本对应的
正常胃粘膜标本中PKC-α蛋白和COX-2蛋白的表达水平。结果（1）胃癌组织中miR-101表达明显低于正常胃粘膜（P<0.05）；
胃癌组织中COX-2及PKC-α蛋白表达高于正常胃粘膜（P<0.05）；（2）胃癌中COX-2蛋白的表达与其分化程度、浸润深度、淋巴
结转移、肿瘤病理分期有关（P<0.05）；胃癌中PKC-α蛋白的表达与肿瘤分期和淋巴转移有关（P<0.05）；（3）胃癌组织中COX-2蛋
白相对表达水平与胃癌组织中PKC-α蛋白表达呈正相关，与胃癌组织中miR-101表达呈负相关，两者相关具有统计学意义（r=
0.531，P<0.05；r=-0.627，P<0.05）。结论miR-101、PKC-α和COX-2可能参与了胃癌的发生发展；miR-101和PKC-α可能是抑制
COX-2在胃癌中表达的新靶点。

Abstract: Objective To investigate the expressions of miR-101, protein kinase C-α (PKC-α), and cycloxygenase-2 (COX-2) in
gastric cancer (GC) tissue and their correlations. Methods RT-qPCR was used to examine miR-101 expression and Western
blotting employed to detect PKC-α and COX-2 expressions in 57 cases of gastric cancer tissues and paired normal gastric
mucosal tissues. Results The gastric cancer tissues showed a significantly lower miR-101 expression (Z=6.102, P<0.05) but
significantly higher expressions of COX-2 (Z=14.436, P<0.05) and PKC-α (Z=6.955, P<0.05) than the normal gastric tissues. The
expression of COX-2 protein was significantly correlated with the degree of differentiation, invasion depth, lymph node
metastasis and TNM stage (P<0.05); PKC-α protein expression was associated with lymph node metastasis and TNM stage (P<
0.05). PKC-α expression was positively correlated (r=0.531, P<0.05) and miR-101 expression negatively correlated (r=-0.627, P<
0.05) with COX-2 expression in gastric cancer tissues. Conclusion miR-101, PKC-α and COX-2 all play a role in the
tumorigenesis and progression of gastric cancer. miR-101 and PKC-α might be new potential therapeutic targets for inhibiting
COX-2 in gastric cancer.