南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (04): 474-.

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ROS kinase fusions are not common in Chinese patients with cholangiocarcinoma

刘鹏敏，武雅君，孙丽，左强，石敏   

  • 出版日期:2013-04-20 发布日期:2013-04-20

  • Online:2013-04-20 Published:2013-04-20

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摘要： 目的探讨胆管癌中ROS融合基因的表达情况。方法收集经甲醛固定和石蜡包埋的I-IV期胆管癌组织，采用RT-PCR对
胆管癌组织进行ROS融合基因（FIG-ROS、SLC34A2-ROS、CD74-ROS）检测。SERPINA1作为参照基因。结果在56个中国人
胆管癌样本中，80.4%（45/56）表达SERPINA1，在这些样本中没有任何一种ROS融合基因表达。结论ROS融合基因在中国胆
管癌病人中不是常见表达基因。

Abstract: Objective To investigate the expressions of different forms of ROS fusions in Chinese patients with
cholangiocarcinoma (CCA). Methods RT-PCR was employed to examine formalin-fixed and paraffin-embedded CCA samples
from stage I-IV patients for detection of ROS fusions involving Fused in Glioblastoma (FIG), solute carrier protein (SLC34A2)
and major histocompatibility complex class II invariant chain (CD74). Serpin peptidase inhibitor clade A member 1
(SERPINA1) was detected as the reference gene. Results In all the 56 CCA samples, 80.4% (45/56) were positive for SERPINA1
expression as evaluable samples. Of these evaluable samples, none expressed the ROS fusions. Conclusion ROS fusions are not
common in Chinese CCA patients.