南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (03): 356-.

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晚期氧化蛋白产物通过氧化应激抑制大鼠成骨细胞增殖及分化

孙楠，杨力，李英彬，程彦臻，张振，蔡德鸿   

  • 出版日期:2013-03-20 发布日期:2013-03-20

Advanced oxidation protein products inhibit proliferation and differentiation of rat osteoblasts through oxidative stress

  • Online:2013-03-20 Published:2013-03-20

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摘要： 目的研究晚期氧化蛋白产物（AOPP）对大鼠成骨细胞增殖、分化及氧化应激的影响及作用机制。方法新生大鼠颅骨酶
解法培养成骨细胞，用CCK-8试剂盒和碱性磷酸酶（ALP）试剂盒检测不同浓度AOPP（50、100、200、400 μg/ml）对成骨细胞增殖
和分化的影响；以2’，7’-二氯二氢荧光素二乙酯为荧光探针检测不同浓度AOPP对成骨细胞OB活性氧含量的影响；实时荧光定
量反转录聚合酶链反应检测成骨细胞ALP、Ⅰ型胶原蛋白、晚期糖基化终末产物受体（RAGE）mRNA表达水平。结果与对照组
相比，AOPP能够显著抑制成骨细胞OB增殖（P<0.01），降低其ALP活性（P<0.01），增加成骨细胞中活性氧的生成（P<0.01），下
调ALP、Ⅰ型胶原蛋白mRNA的转录（P<0.05），并增加RAGE mRNA的表达（P<0.05）。结论AOPP可抑制成骨细胞增殖及分
化，可能与上调RAGE mRNA表达，诱导成骨细胞产生活性氧有关。

Abstract: Objective To investigate the effect of advanced oxidation protein products (AOPP) on proliferation, differentiation,
and oxidative stress in rat osteoblasts. Methods The cell proliferation and differentiation of osteoblasts isolated from neonatal
SD rat skull were evaluated following treatment with different concentrations (50, 100, 200, and 400 μg/ml) of AOPP using
CCK-8 kit and ALP assay kit, respectively. The levels of reactive oxygen species (ROS) in the treated cells were analyzed using
2’, 7’-dichlorofluorescin diacetate, and the transcription levels of ALP, collagen I and RAGE were assessed using real-time PCR.
Results Compared with the control group, AOPP-treated osteoblasts showed obviously inhibited proliferation and
differentiation with down-regulated expressions of ALP and collagen I and increased ROS production and RAGE expression.
Conclusion AOPP can inhibit the proliferation and differentiation of rat osteoblasts partially by up-regulating RAGE and
inducing ROS production.