南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (03): 322-.

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山楂酸预处理对皮层神经元氧糖剥夺损伤的影响

黄柏胜，罗洁，韩仰，李思敏，刘发益，邬力祥   

  • 出版日期:2013-03-20 发布日期:2013-03-20

Protective effect of maslinic acid preconditioning against oxygen-glucose deprivationinduced injuries in embryonic rat cortical neurons

  • Online:2013-03-20 Published:2013-03-20

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摘要： 目的探讨山楂酸预处理对大鼠大脑皮层神经元氧糖剥夺（oxygen-glucose deprivation,OGD）损伤的影响。方法原代培
养SD大鼠15~17 d胎鼠皮层神经元，预先用不同浓度的山楂酸(0.1、1、10 μmol/L)预处理，观察在OGD培养状态下细胞的功能
状态和损伤情况。以四甲基偶氮唑盐还原试验检测细胞活力，乳酸脱氢酶(LDH)漏出率作为细胞损伤指标，以Western blot检测
凋亡相关蛋白Bax的表达。结果OGD引起细胞活力下降，LDH漏出率增高，与正常组细胞相比均有非常显著意义；两组细胞
经不同浓度山楂酸（0.1、1、10 μmol/L）预处理后，分别与未经山楂酸预处理的细胞比较结果显示：OGD组细胞LDH漏出率在山
楂酸浓度为1、10 μmol/L时降低，而正常组细胞LDH漏出率在山楂酸浓度为10 μmol/L明显降低；两组细胞的存活率均在给予
山楂酸预处理浓度为1、10 μmol/L 时明显升高。Western blot 结果显示凋亡相关蛋白Bax的表达随着山楂酸的浓度增加而下
降，以山楂酸浓度为10 μmol/L时更明显。结论山楂酸对体外培养的大鼠大脑皮层神经元OGD损伤有保护作用，可能与凋亡
相关蛋白Bax表达降低有关。

Abstract: Objective To investigate the effect of maslinic acid preconditioning against injuries of rat cortical neurons induced
by oxygen-glucose deprivation (OGD). Methods The cortical neurons were isolated from Sprague-Dawley rat embryos at
15-17 days of gestation for primary culture. The cortical neurons were incubated with different concentrations (0.1, 1, and 10
μmol/L) of maslinic acid prior to OGD. The cell damage and viability were evaluated for lactate dehydrogenase (LDH) leakage
and using MTT assay, respectively, and the expression of Bax protein was detected using Western blotting. Results OGD
significantly increased LDH release rate and decreased the viability of the cells. After preconditioning with maslinic acid (1
and 10 μmol/L), LDH leakage rate was decreased and cell the viability increased in cells exposed to OGD. Western blotting
showed that Bax expression in the cells decreased as maslinic acid concentrations increased. Conclusion Pretreatment with
maslinic acid can protect cultured embryonic rat cortical neurons against OGD-induced injury possibly in relation to decreased
expression of Bax.